DEVELOPMENT AND VALIDATION OF A RAPID RP-HPLC METHOD FOR ESTIMATION OF SPARFLOXACIN IN TABLET DOSAGE FORM
AbstractThe study describes development and subsequent validation of a reverse phase (RP) high-performance liquid chromatography (HPLC) method for the estimation of sparfloxacin (SPR) in tablet dosage form. The liquid chromatographic separation was achieved isocratically using a mobile phase composed of phosphate buffer (5.3 mM) and acetonitrile in the ratio of 60:40 (v/v) adjusted to pH 3.5 using dilute phosphoric acid. The analysis was carried out using X terra column (4.6 ´ 150 mm, 5µm, C18) at a flow rate of 0.5 ml/min and the UV detection at 298 nm. The method was statistically validated for accuracy, precision, linearity, ruggedness, robustness and selectivity. The linearity of the proposed method was investigated in the range of 4 to 24 µg/ml (r2 =0.999). Quantitative and recovery studies of the dosage form were also carried out and analyzed, the % RSD from recovery studies was found to be within the limit. Due to simplicity, rapidity and accuracy of the method we believe that the method will be useful for routine quality control analysis.
Article Information
34
563-567
345KB
1313
English
IJPSR
Ashok Kumar Bera , Amit Kumar De and Biswajit Pal*
Department of Chemistry, St. Paul’s C. M. College, 33/1 Raja Rammohan Roy Sarani, Kolkata 700009, West Bengal, India
palbiswajit@yahoo.com
27 September, 2013
29 November, 2013
14 January, 2014
http://dx.doi.org/10.13040/IJPSR.0975-8232.5(2).563-67
01 February, 2014
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