DEVELOPMENT AND VALIDATION OF A REVERSED PHASE HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF OLMESARTAN MEDOXOMIL AND HYDROCHLOROTHIAZIDE IN COMBINED TABLET DOSAGE FORMAbstract
A simple reversed phase HPLC method has been developed for the simultaneous determination of olmesartan medoxomil in combination with hydrochlorothiazide. The method was based on reversed phase liquid chromatography using a Grace Smart RP C18 column (250 × 4.6 mm, 5µ) with UV detection at 256 nm. The mobile phase consisting of acetonitrile and triethylamine buffer adjusted to pH 2.7 in a ratio of (30:70, v/v) and at a flow rate of 2 ml/min. The method was linear over the concentration range for olmesartan medoxomil 4.0-40.0 μg/mL and for hydrochlorothiazide 5.0-50.0 μg/mL. The recoveries of olmesartan medoxomil and hydrochlorothiazide were found to be in the range of 99.25-100.46% and 99.31-102.13% respectively. The method was validated and was successfully employed for the analysis of pharmaceutical formulations containing olmesartan medoxomil and hydrochlorothiazide in combined tablet dosage form.
Moynul Hasan*, Abdullah Al Masud and Jamiuddin Ahmed
Department of Pharmacy, Dhaka International University, Banani, Dhaka, Bangladesh
19 July, 2010
07 October, 2010
01 November, 2010
01 December, 2010