DEVELOPMENT AND VALIDATION OF A SIMPLE UV SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF LEVOFLOXACIN BOTH IN BULK AND PHARMACEUTICAL FORMULATIONAbstract
The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV-Visible spectrophotometric method for the estimation of levofloxacin in bulk and pharmaceutical formulation. The solvent used throughout the experiment was 0.1N NaOH. Absorption maximum (λmax) of the drug was found to be 288 nm. The quantitative determination of the drug was carried out at 288 nm and Beer’s law was obeyed in the range of 2-12μg/mL. The method was shown linear in the mentioned concentrations having line equation y = 0.0704x + 0.0034 with correlation coefficient R2of 0.999. The recovery values for levofloxacin ranged from 99.92% – 100.33%. The relative standard deviation of six replicates of assay was less than 2%.The percent relative standard deviation (RSD %) of interday precision range was 0.469 – 0.925 % and intraday precision range was 0.685 – 0.713%. The limit of detection and limit of quantification was 0.087μg/mL and 0.164μg/mL. The percent relative standard deviation of robustness and ruggedness of the method was 0.136 – 0.213%. Hence, proposed method was precise, accurate and cost effective. This method could be applicable for quantitative determination of the bulk drug as well as dosage formulation.
Most. Umme Bushra*, Md. Saddam Hossain , Kh. Tanvir Ahmed , Jeb-Un Nesa and Md. Firoz Mahmud
Department of Pharmacy, Manarat International University, Mirpur, Dhaka, Bangladesh
25 December, 2013
30 April, 2014
12 May, 2014
01, June 2014