DEVELOPMENT AND VALIDATION OF DUAL WAVELENGTH UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF AMBROXOL HYDROCHLORIDE AND DESLORATADINE HYDROCHLORIDE IN THEIR COMBINED TABLET DOSAGE FORMAbstract
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical dual wavelength spectrophotometric method for the simultaneous determination of Ambroxol Hydrochloride and Desloratadine Hydrochloride in combined tablet dosage form. The principle for dual wavelength method is “the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest”. The method was based on determination of Ambroxol Hydrochloride at the absorbance difference between 253.2 nm and 258.5 nm and Desloratadine Hydrochloride at the absorbance difference between 301.2 nm and 314 nm. The linearity was obtained in the concentration range of 5 – 75μg/ml for both Ambroxol Hydrochloride and Desloratadine Hydrochloride. The method was successfully applied to pharmaceutical dosage form because no interference from the tablet excipients was found. The suitability of these methods for the quantitative determination of Ambroxol Hydrochloride and Desloratadine Hydrochloride was proved by validation and recovery study. The proposed methods were found to be simple and sensitive for the routine quality control application of Ambroxol Hydrochloride and Desloratadine Hydrochloride in pharmaceutical tablet dosage form.
E.A. Sharma* and N. J. Shah
Pharmaceutical Chemistry Department, Indubhai Patel College of Pharmacy and Research Centre, Dharmaj, Petlad- Khambhat Road, Anand, Gujarat, India
04 April, 2012
20 May, 2012
22 July, 2012
01 August, 2012