DEVELOPMENT AND VALIDATION OF HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHIC METHODFOR LAFUTIDINE IN BULK AND TABLET DOSAGE FORM
AbstractA simple, precise and sensitive High Performance Liquid Chromatographic method has been developed for the quantitative estimation of Lafutidinein bulk and tablet dosage form. The method was performed on Silicagel 60 F254 aluminum plate. A Camag HPTLC system comprising of Camag Linomat IV semiautomatic sample applicator, Camag TLC Scanner 3, Camag twin-trough chamber (10×10 cm) and Camag CATS 4 software, As mobile phase Ethyl acetate: Toluene: Methanol: Ammonia (6:2.5:1.5:0.1%v/v) was utilized to develop the Chromatographic method. Lafutidinegave separation at Rf value of 0.45±0.03 and scanned at 273 nm. The calibration curve response was observed between 400-1400ng/spot. The linear regression data showed good linear relationship with R2 value of 0.99895. The method was validated in terms of repeatability, precision, accuracy, and specificity according to ICH guidelines. The LOD and LOQ were found to be 19ng/Spot and 59 ng/spot respectively. The developed method was successfully used for assay of Lafutidine in tablet formulation.
Article Information
36
921-925
978 KB
196
English
IJPSR
Vidhi Kotadiya * and Jaya Patel
Department of Quality Assurance, K. B. Raval College of Pharmacy, Kasturinagar, Shertha, Gandhinagar, Gujarat, India.
vidhidave1988@gmail.com
08 August 2023
24 October 2023
30 December 2023
10.13040/IJPSR.0975-8232.15(3).921-25
01 March 2024
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