DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF RIFAMPICIN AND ISONIAZID IN BULK AND TABLET DOSAGE FORM
AbstractA new HPLC method has been developed and validated for the simultaneous estimation of Rifampicin and Isoniazid, offering a reliable and efficient approach for their analysis. The method employs a mobile phase composed of pH 3.5 phosphate buffer, methanol, and water in a 45:30:25 ratio. It demonstrates a linear response for both drugs within specific concentration ranges, allowing for accurate quantification. Detection is performed at 239 nm, with retention times of 2.8 minutes for Rifampicin and 3.7 minutes for Isoniazid. Accuracy studies have shown excellent recovery rates within ICH guidelines, ensuring the reliability of the results. Notably, the method exhibits high precision with RSD values within acceptable limits, indicating its reproducibility. It has also been found to be simple, rapid, robust, and reproducible, making it well-suited for routine use. This validated HPLC method holds significant potential for both pharmaceutical and clinical applications, enabling accurate and efficient analysis of Rifampicin and Isoniazid in bulk and tablet dosage forms.
Article Information
19
1441-1448
689 KB
262
English
IJPSR
Krutanjali Nikumbh *, Kiran Dhamak and Charushila Bhangale
Department of Pharmaceutical Quality Assurance, PRES College of Pharmacy (For Women’s), Chincholi, Nashik, Maharashtra, India.
krutanjalinikumbh01@gmail.com
22 October 2023
10 January 2024
28 February 2024
10.13040/IJPSR.0975-8232.15(5).1441-48
01 May 2024
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