DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR SIMULTANEOUS ESTIMATION OF ROSUVASTATIN CALCIUM AND ASPIRIN IN CAPSULE DOSAGE FORMAbstract
This paper described validated high performance thin layer liquid chromatographic (HPTLC) method for estimation of Rosuvastatin Calcium (ROSU) and Aspirin (ASP) in capsule dosage form. The method involved separation of components by TLC on a precoated silica gel 60 F254 using a mixture of n-Hexane: Acetone: Ethyl acetate: Formic acid (6:3:1:0.2 v/v) as a mobile phase. Detection of spots was carried out at 240 nm for Rosuvastatin Calcium and Aspirin both. The mean retardation factor for Rosuvastatin Calcium and Aspirin were found to be 0.31±0.02 and 0.60±0.02, respectively. The linear regression data for the calibration plots showed good linear relationship with r2 value 0.995 and 0.995 in the concentration range of 500-1000 ng/spot and 3750-7500 ng/spot for Rosuvastatin calcium and Aspirin, respectively. The developed method was validated as per ICH Guidelines.
Pallavi H. Tank*, K. R. Vadalia, Z. R. Dedania
Shree H. N. Shukla Institute of Pharmaceutical Education & Research, B/H-Marketing Yard, Nr. Lalpari Lake, Amargadh (Bhichari), Rajkot- 3600, Gujarat, India
12 June, 2012
24 September, 2012
26 September, 2012
01 October, 2012