DEVELOPMENT AND VALIDATION OF SECOND-ORDER DERIVATIVE UV SPECTROPHOTOMETRIC ESTIMATION OF FAVIPIRAVIR ANTIVIRAL DRUG

Objectives: The present study aims to develop a simple, precise, accurate, and reproducible spectrophotometric Second order derivative method for estimating Favipiravir in bulk and pharmaceutical dosage form. Method: Acetonitrile was used as an organic solvent. The maximum absorption of the drug was found to be at 323nm. The developed method was validated and applied for the estimation of Favipiravir by using a spectrophotometric second-order derivative. Results: The linearity was established in the 5 to 25µg/ml concentration range for favipiravir with a correlation coefficient (r²) of 0.9991, respectively. Percent recovery studies were obtained in the range of 99.55-101.25%. Both interday and intraday precision was found to be satisfactory. The limit of detection and quantitation were found to be 1.5µg/ml and 1.2µg/ml, respectively. The method was statistically validated according to ICH guidelines for linearity, accuracy, and precision. The relative standard deviation was found to be less than 2%. Conclusion: The proposed method is simple to use, affordable and cost-effective. It can be successfully applied to estimate favipiravir in bulk and pharmaceutical dosage form and used for routine analysis.