DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ESTIMATION OF ANTIFUNGAL DRUGS IN BULK AND PHARMACEUTICAL DOSAGE FORM BY CHROMATOGRAPHIC METHOD USING HPLC
AbstractA new simple, accurate, rapid, selective and robust high-pressure liquid chromatography (HPLC) method was developed and validated for simultaneous estimating itraconazole and Terbinafine in bulk and marketed formulation. Acetonitrile and double distilled water was used as a mobile phase for chromatographic separation and estimation on C18 Targetsil column with dimensions 250×4.6 mm in the ratio of 90:10 v/v at flow rate of 1.0 ml/min. The detection was carried out with U.V. Visible detector (Shimadzu SPD10A) at 225 nm. The retention time for itraconazole (6.1 min) and for Terbinafine (9.8min) was obtained. The linearity range for itraconazole and Terbinafine was 1-8μg/ml with a coefficient of linear regression 0.996 and 0.992, respectively. The method was validated in accordance with the requirements of the International Conference on Harmonization (ICHQ2 (R1) 2005) guidelines for accuracy, precision, LOD & LOQ, linearity and robustness.
Article Information
52
4102-4113
841 KB
266
English
IJPSR
Shweta Singh * and Virendra Kumar Sharma
Department of Medicinal Chemistry, School of Pharmacy, LNCT University, Bhopal, Madhya Pradesh, India.
bpart0001@gmail.com
28 November 2022
06 February 2023
11 June 2023
10.13040/IJPSR.0975-8232.14(8).4102-13
01 August 2023
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