DEVELOPMENT AND VALIDATION OF STABILITY INDICATING ASSAY FOR SIMULTANEOUS DETERMINATION OF PENTAPRAZOLE, DICLOFENAC, CHLOROXAZONE IN PHARMACEUTICAL DOSAGE FORM BY USING RP-HPLC

Objective: A simple, accurate, precise method was developed for the simultaneous estimation of the Chlorzoxazone, Pantoprazole, and Diclofenac in solid dosage form. Method: Chromatogram was run through BDS C18 150 × 4.6 mm, 5 m. Mobile phase containing 0.01% KH₂PO₄ and Acetonitrile in the ratio of 52:48 v/v was pumped through column at a flow rate of 1.0 ml/min. The buffer used in this method was 0.01%. KH₂PO₄, Temperature was maintained at 30 °C. The optimized wavelength for Chlorzoxazone, Pantoprazole, and Diclofenac was 229.0 nm. Results: Retention time of Chlorzoxazone, Pantoprazole, and Diclofenac were found to be 2.229 min, 2.958 min, and 3.568 min. % RSD of system precision for Chlorzoxazone, Pantoprazole and Diclofenac were and found to be 0.9, 0.6 and 0.6, respectively. % RSD of method precision for Chlorzoxazone, Pantoprazole and Diclofenac were and found to be 0.6, 1.0 and 0.7 respectively. % recovery was obtained as 99.86%, 100.07% and 99.70% for Chlorzoxazone, Pantoprazole, and Diclofenac respectively. LOD values are obtained from regression equations of Chlorzoxazone, Pantoprazole and Diclofenac were 0.14 ppm, 0.24 ppm, 1.83 pm, and LOQ values are obtained from regression equations of Chlorzoxazone, Pantoprazole and Diclofenac were 0.42 ppm, 0.72 pm, 5.53 ppm respectively. The regression equation of Chlorzoxazone was y = 8321.9.x + 1397.8Pantoprazolee was y = 9806.1x + 6071.7 and of Diclofenac was y = 2575x + 4338.4. Conclusion: Retention times are decreased so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.