DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF EMPAGLIFLOZIN AND METFORMIN HYDRO-CHLORIDE IN COMBINED DOSAGE FORMAbstract
Two simple spectrophotometric methods have been developed for the simultaneous estimation of Empagliflozin and Metformin hydrochloride from the tablet dosage form. Method-I simultaneous equation method involves the measurement of absorbances at two wavelengths 224 nm (λmax of Empagliflozin) and 233nm (λmax of Metformin hydrochloride) using Methanol and Water as diluent. Method – II Absorption ratio method involves the measurement of absorbances at two wavelengths 233 nm (λmax of Metformin Hydrochloride) and 266 nm (λmax of Isobestic point). The linearity lies between 0.1-25 µg/ml for Empagliflozin and 0.5-25 µg/ml Metformin hydrochloride in both methods. The accuracy and precision of the methods were determined and validated statistically. The two methods exhibited good reproducibility and recovery with a relative standard deviation of <2%. Both the methods were found to be rapid, specific, precise, accurate, and reproducible and can be successfully applied for the routine analysis of Empagliflozin and Metformin hydrochloride in a combined dosage form.
P. Ashwini, J. Archana * and M. Sumakanth
RBVRR Women’s College of Pharmacy, Osmania University, Hyderabad, Telangana, India.
18 June 2019
28 August 2019
08 February 2020
01 May 2020