DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHODS FOR ESTIMATION OF RILPIVIRINE IN BULK AND PHARMACEUTICAL FORMULATION
AbstractIn the present research work, four simple, precise, accurate and economical methods of UV spectroscopy have been developed for the estimation of Rilpivirine in bulk and pharmaceutical formulation. Method A involves estimation of Rilpivirine using low cost solvent, 0.01N HCl by zero order spectroscopy at an absorption maximum of 280nm. Method B involves Area under Curve method which involved calculation of integrated value of absorbance with respect to wavelength between two wavelengths selected, 275nm and 285nm respectively. Method C involves first order derivative technique for the same at 264nm. Method D involves second order derivative technique for the same at 237nm. The developed methods were found to be linear in the concentration range of 0.5-7.5μg/mL with correlation coefficient (R2) of 0.9998. The mean percentage label claim of tablets of Rilpivirine estimated by proposed methods was within the acceptable range. The developed methods were validated by following the analytical performance parameters suggested by ICH. All the validation parameters were within the acceptable range. As economical solvent is used, these methods can be used for routine analysis of Rilpivirine in bulk and pharmaceutical formulation.
Article Information
25
483-489
454KB
1958
English
IJPSR
Masthanamma Sk* and Alekhya Gottumukkala
Department of Pharmaceutical Analysis, University College of Pharmaceutical Sciences, Acharya Nagarjuna University, Nagarjuna nagar, Guntur, Andhra Pradesh, India
masthanamma.sk@gmail.com
20 September, 2013
23 October, 2013
11 January, 2014
http://dx.doi.org/10.13040/IJPSR.0975-8232.5(2).483-89
01 February, 2014
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