DEVELOPMENT OF A SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF CEPHALEXIN USING SODIUM 1, 2-NAPHTHOQUINONE-4-SULFONATE AND CENTRAL COMPOSITE DESIGN
HTML Full TextReceived on 14 August, 2013; received in revised form, 13 October, 2013; accepted, 26 December, 2013; published 01 January, 2014
DEVELOPMENT OF A SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF CEPHALEXIN USING SODIUM 1, 2-NAPHTHOQUINONE-4-SULFONATE AND CENTRAL COMPOSITE DESIGN
Mohsen Irandoust, Masoud Shariati-Rad* and Mina Khodabakhshi
Department of Analytical Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran
ABSTRACT: A spectrophotometric method for determination of cephalexin is proposed. This method is based on the formation of a colored compound in the reaction of cephalexin with sodium 1,2-naphthoquinone-4-sulfonate (NQS). The effects of various experimental parameters in this reaction were investigated and optimized using central composite design (CCD). Twelve experiments with two factors and five levels for each factor were designed. These factors include concentration of NQS and pH. A full-quadratic polynomial equation between the absorbance as the response and the studied parameters was established. After removing the non-significant terms from the model, response surface method was used to obtain the optimum conditions. The optimum values of factors were 20.0×10^{-4} M for concentration of NQS and 9.6 for pH. The maximum absorption wavelength and the value of absorptivity of the colored product were 475 nm and 1.79×10^{3} L mol^{−1} cm^{−1}, respectively. The absorbance of the reaction product obeys Beer’s law in the range 1.5-34.0 mgL^{-1}. The detection limit of the method was 0.43 mgL^{-1}. This method is rapid and simple, and can be used for the determination of cephalexin in formulations (capsules and injections). Recovery of 98.8% was obtained in application of the method to the real samples.
Keywords: |
Cephalexin, 1,2-naphthoquinone-4-sulfonate, Central composite design, Formulation, Recovery
INTRODUCTION:Cephalexin (7-[(aminophenyl acetyl) amino]-3-methyl-8-oxo-5-thia- l-azabicyclo [4.2.0]oct-2-ene-2-carboxyliaccid) is a semi-synthetic β-lactam antibiotic which belongs to the group of cephalosporin antibiotics. They have antibacterial action against gram-positive and gram-negative bacteria. Cephalexin is a potent cephalosporin and exhibits a broad spectrum of antibiotic activity. ^{ }
A great variety of methods have been reported to determine cephalexin, including microbiological, ^{1, 2} spectrophotometric ^{3-5}, fluorimetric, ^{6, 7}, HPLC ^{8-16}, polystyrene-divinylbenzenecolumn (PSDVB) ^{17}, high speed liquid chromatographic (HSLC) ^{18}, high-pressure thin layer chromatography (HPTLC)^{ 19-23}, immunoanalysis ^{24, 25}, alternative assay technique ^{26} and electrochemical ^{27-30}.
The microbiological assay is highly sensitive and specific but it is time-consuming and expensive. The proposed polarographic and fluorimetric techniques for determination of cephalexin require tedious procedures including solvent extractions and chemical pretreatment. HPLC methods can be used to determine the cephalexin in pharmaceutical dosage forms, but it has poor reproducibility.
PSDVB methods are related to the separation of drug from impurities. Other methods of analysis available in the literature are based on electrochemical techniques. These sophisticated instrumental methods are not suitable for counterfeit drug assay as they are expensive.
The present work provides a reliable, simple and less time-consuming method for the determination cephalexin and need no separation of drug from impurities. However, the use of sodium 1, 2-naphthoquinone-4-sulfonate (NQS) as a chemical derivative chromogenic reagent for the determination of cephalexin by a condensation reaction has not been reported so far.
EXPERIMENTAL:
Apparatus: An agilent 8453 spectrophotometer was used for spectrophotometric measurements in a 1 cm quartz cells. The pH values were measured by a Jenway model 3345 pH-meter. A Huber polystat model CC3 thermostat was employed for temperature controlling.
Reagents: All chemicals were of analytical grade and used as received without any further purification. These were NQS (Merck, >99%), sodium carbonate (Na_{2}CO_{3}.H_{2}O) and Cephalexin. Double-distilled water was used in all experiments.
Two different commercial capsules containing 500 and 250 mg cephalexin per capsules from Tehranchimie pharmaceutical company were purchased and used as real samples.
Solutions: A 1.0×10^{-3 }M standard solution of cephalexin was prepared by dissolving 0.035g of drug in double-distilled water and diluting it to100 mL.
A 4.0×10^{-3 }M standard solution of sodium NQS was prepared by dissolving 0.026 g of solid in double-distilled water and diluting it to 25 mL. NQS solutions were freshly prepared for experiment any stage.
Buffer solutions of different pH were prepared by adding concentrated HCl or NaOH to the 5.0×10^{-3 }M solution of Na_{2}CO_{3}.
Procedure for recording spectra: A 1.50 mL Na_{2}CO_{3}-NaHCO_{3} buffer solution of pH 9.6, 1.20 mL NQS and aliquots of cephalexin stock solutions were transferred sequentially into a 10.00 mL standard flask, diluted to volume with double-distilled water and mixed well. The reaction was allowed to proceed at room temperature (25.00 ±1 °C) for 30 min. The absorbance of the solution was measured at 475 nm against a reagent blank prepared with the same reagents concentration, but no cephalexin.
Preparation of the real capsule solutions: The contents of ten capsules were weighed, finely powdered, and mixed thoroughly. A weight of the powder equivalent to the weight of the content of a capsule (containing 500 or 250 mg cephalexin) was transferred into a 1000 mL volumetric flask, dissolved in about 50 mL water, sonicated for 15 min, filtered and diluted to the mark with double-distilled water, and mixed well. The resulting solution was analyzed based on the “Procedure for recording spectra”.
RESULT AND DISCUSSION:
Reaction between cephalexin and NQS: NQS reacts with amino group of cephalexin to form a product. λ_{max} of the absorbance of the product is at 475 nm. The reaction has been shown in Scheme 1.
SCHEME 1: REACTION BETWEEN CEPHALEXIN AND NQS
Absorption spectrum: According to the procedure, the absorption spectrum of the product I produced by the reaction between cephalexin and NQS was recorded and shown in Fig. 1. As can be seen in Fig. 1, the maximum absorption wavelength of product I is at 475 nm and that for NQS is located at wavelengths lower than 400 nm. Obviously, cephalexin has no absorption in the range of 400–550 nm.
FIG. 1: ABSORBANCE SPECTRUM OF (A) CEPHALEXIN AGAINST WATER AND BUFFER BLANK, (B) NQS AGAINST REAGENT BLANK AND (C) PRODUCT I AGAINST REAGENT BLANK. Conditions: concentration of cephalexin 3.5 mg.L^{-1}, concentration of NQS 20.0×10^{-4 }M and pH = 9.6.
Optimization of reaction conditions: Optimizing refers to improving the performance of a system, a process, or a product in order to obtain the maximum benefit from it. The term optimizationhas been commonly used in analytical chemistry as a means of discovering conditions at which a procedure produces the best possible response. The experimental design is a method which can lead to this goal. The effect of NQS concentration and pH value on the formation of the reaction product was investigated by carrying out the reaction for different NQS concentrations (different volumes of 4.0×10^{-3} M NQS solution) and pH (Table 1). Experiments were carried out at 25°C and reaction time was 30 min.
TABLE 1: EXPERIMENTS DESIGNED BASED ON THE CCD FOR THE REACTION OF CEPHALEXIN WITH NQS
Experiment number | pH | C (M) |
1 | 9.0 | 20.0×10^{-4} |
2 | 9.0 | 12.5×10^{-4} |
3 | 11.0 | 12.5×10^{-4} |
4 | 7.0 | 12.5×10^{-4} |
5 | 7.6 | 18.0×10^{-4} |
6 | 10.4 | 7.2×10^{-4} |
7 | 9.0 | 12.5×10^{-4} |
8 | 7.6 | 7.2×10^{-4} |
9 | 9.0 | 5.0×10^{-4} |
10 | 9.0 | 12.5×10^{-4} |
11 | 10.4 | 18.0×10^{-4} |
12 | 9.0 | 12.5×10^{-4} |
- Design of experiments: By using central composite design (CCD), twelve experiments (including four replicates at the central point) were designed. The factors (variables) were: initial concentration of NQS (C) and pH value. Although the initial concentration of cephalexin might be important, this factor was kept constant at a value of 1.0×10^{-5 }M. For each factor, five levels were defined. These values were designated by the codes: –2, –1, 0, +1 and +2 and are given in Table 2.
TABLE 2: THE FACTORS, THEIR CODES AND THE REAL EXPERIMENTAL VALUES USED IN THE CENTRAL COMPOSITE DESIGN
Factor | Name | Coded levels | ||||
-2 | -1 | 0 | +1 | +2 | ||
F_{1} | C (M) | 5.0×10^{-4} | 7.2×10^{-4} | 12.5×10^{-4} | 18.0×10^{-4} | 20.0×10^{-4} |
F_{2} | pH | 7.0 | 7.6 | 9.0 | 10.4 | 11.0 |
- Analysis of data: After performing the experiments, the net absorbance for each experiment was calculated by ΔA= A_{s475 }-A_{b475 }where A_{bλ} and A_{sλ} are the absorbances of the blank and sample, respectively at the indicated wavelength.
The values of the difference between the absorbance of the blank and the absorbance of sample for each experiment are responses and are given in Table 3.
TABLE 3: RESULTS OF THE EXPERIMENTS DESIGNED BY CCD (Table 1)
Run No. | ΔA_{experimental} | ΔA_{predicted} |
1 | 0.4267 | 0.4500 |
2 | 0.2518 | 0.2880 |
3 | 0.1796 | 0.2880 |
4 | 0.2912 | 0.2880 |
5 | 0.4004 | 0.4070 |
6 | 0.1901 | 0.1730 |
7 | 0.2724 | 0.2880 |
8 | 0.1767 | 0.1730 |
9 | 0.1339 | 0.1250 |
10 | 0.3219 | 0.2880 |
11 | 0.4777 | 0.4070 |
12 | 0.3376 | 0.2880 |
The closeness of the responses of the four replicate experiments can be a sign of the precision of the experiment process. The relation between the collected response and the variables conforms to the following polynomial equation (full quadratic model):
Y = b_{0} + b_{1}F_{1} + b_{2}F_{2} + b_{11}F_{1}F_{1} + b_{22}F_{2}F_{2 }+ b_{12}F_{1}F_{2 } …….. (1)
where Y is a response variable ΔA, b_{i} are regression coefficients for linear effects, b_{ik} are regression coefficients for quadratic effects and F_{i} are coded experimental levels of the variables. The analysis of variance (ANOVA) and least squares were used to evaluate the statistical significance of the variables and construct the model. The ANOVA consists of determining which factor(s) significantly affect the response. The significance and the magnitude of the estimated coefficients of each variable and all their possible interactions in the model (Eq. (1)) were determined and reported in Table 4.
Such coefficients for each variable represents the improvement in the response, that is, to expect as the variable setting is changed from low to high level. Four replicates of the central points were performed to estimate the experimental error. In order to show the fitness of the model, regression coefficient (R) maybe be used. However, the adjusted regression coefficient (R_{adj}) is a better criterion than the absolute regression coefficient. Since the regression coefficient (R) always decreases when a regression variable is eliminated from the model, in statistical modelling, the R_{adj}, which takes the number of regression variables into account, is usually selected.
Hence, R and R_{adj }together are very convenient to obtain a quick impression of the overall fit and the predictive power of a constructed model. R and R_{adj }values of the model are high. The values of these parameters indicate that the model explains more than 85% of the variations in the experimental data. The parameter F of the regression is also high which indicates that the model is reliable in prediction of the response based on the values of the factors studied.
In order to find the important factors and build a model to optimize the procedure, initially the full quadratic model including all terms in Eq. (1) was employed. Then by the back elimination process, those terms which were not significant enough were eliminated. These terms included the factors or the interactions which had no significant effect on the response. Each term with a p-value greater than 0.05 was removed from the main equation. As can be inferred from Table 4, initial concentration of NQS and the constant term of the model are significant at the 95% confidence level (p-value lower than 0.05). Although pH may be important in the reaction of the amines with NQS, the ANOVA results show that pH is not a significant factor. This may be due to the result of the range of pH studied. Moreover, interaction terms are not important.
TABLE 4: ANALYSIS OF VARIANCE (ANOVA) OF THE EXPERIMENTAL DATA
Term | Coefficient | t | p | |
Constant | 0.296 | 10.605 | 0.000 | |
pH | -0.012 | -0.441 | 0.674 | |
C | 0.162 | 5.880 | 0.001 | |
pH×pH | -0.036 | -0.803 | 0.453 | |
C×C | 0.011 | 0.258 | 0.805 | |
pH×C | 0.033 | 0.594 | 0.574 | |
R | 92.58% | |||
R_{adj} | 85.91% | |||
F regression | 47.04 |
- Response surfaces and selection of optimized values: Response surface methodology (RSM) is a statistical method being useful for the optimization of chemical reactions and/or industrial processes and widely used for experimental design. Whenever multiple system factors may influence the responses, RSM can be utilized to assess the relationship between dependent (response) and independent variables (factors) as well as to optimize the relevant processes.
After analysis of the data, the response surface of the full quadratic model between the response and the factors was depicted. Viathe surface, the relations between the response and the effective factors are graphically given. The surface is shown in Fig. 2. The optimized ranges for each factor that leads to the best response (the highest absorbance intensity) were extracted from this surface. The optimum values of variables were calculated as: 20.0×10^{-4} M for C and 9.6 for the reaction pH.
FIG. 2: RESPONSE SURFACE FOR THE VARIATION OF THE FACTORS CONCENTRATION OF NQS (C) AND pH
Effect of factors: The optimum values of the factors show that higher values of concentration of NQS and intermediate pH are suitable for the reaction between cephalexin and NQS. The coefficient of the concentration of NQS (C) is positive (Table 4) and it is the largest ones among the coefficients except the constant term. This indicates the importance of the concentration of NQS in the reaction studied. From Fig. 2, the significant effect of concentration of NQS (C) on the response is clearly observed. On the other hand, the coefficient of pH is negative and it is one of the smallest ones in the model. Therefore, pH is not as important as the concentration of NQS in the reaction studied. This can be simply inferred from Fig. 2. With variation of pH, response changes only slightly. However, the overall effect of pH is such that the production of the colored product favors in the intermediate studied pHs.
Calibration: Under the optimum conditions, a linear relationship between concentration of cephalexin and ΔA was obtained in the range of 1.5–34.0 mg.L^{-1}. In addition, based on the absorbance and concentration of the cephalexin, molar absorptivity of product I at 475 nm was ε_{475} = 1.79×10^{3} L mol^{-1}cm^{-1}. Statistical analyses of the calibration have been collected in Table 5.
TABLE 5: STATISTICAL ANALYSIS OF THE CALIBRATION BY THE PROPOSED METHOD
Parameter | Parameter |
λ_{max} (nm) | 475 |
Linear range (mg.L^{-1}) | 1.5-34.0 |
slope (n=11) | 0.0090 |
intercept (n=11) | 0.0900 |
Correlation coefficient (R) | 0.9960 |
Detection Limit (mg.L^{-1}) | 0.4316 |
Standard deviation of blank | 0.0022 |
Standard deviation of slope | 0.0008 |
Standard deviation of regression | 0.0305 |
Analysis of the real samples: The applicability of the method was assayed by analyzing two different pharmaceutical formulations. The assays were carried out as described under the preparation of the real capsule solutions. In all cases, the concentration of cephalexin was obtained by reaction of the real samples with NQS in the optimum conditions and using the standard calibration graph.
The results have been shown in Table 6. Accuracy of the proposed method is evident from the percent recoveries very close to 100%. The method is also precise since the RSD% values are below 3%.
TABLE 6: RESULTS OF DETERMINATION OF CEPHALEXIN IN PHARMACEUTICAL FORMULATIONS BY THE PROPOSED METHOD
Sample no. | Real sample (mgL^{-1}) | Mean found (mgL^{-1}) (n=5) | RSD (%) | Recovery (%) |
(Cephalexin Tehranchemie 500 mg capsule) | 10 | 9.88 | 2.6 | 98.8 |
(Cephalexin Tehranchemie 250 mg capsule) | 10 | 9.95 | 1.5 | 99.5 |
Comparison of the proposed method with the reported spectrophotometric method: Statistical analysis of the results obtained (Table 7) indicates that lower limit of the linear range of the proposed method is smaller and molar absorptivity of product I at 475 nm is ε_{475} = 1.79×10^{3 }L mol^{-1 }cm^{-1}. Linear range or the reported spectrophotometric method ^{5} is relatively wide. The relative standard deviation (RSD) of the two methods is less than 2% and recoveries are over 98%.
TABLE 7: ANALYTICAL DATA FOR DETERMINATION OF CEPHALEXIN BY THE PROPOSED METHOD AND THE REPORTED SPECTROPHOTOMETRIC METHOD.
Linear range (mg.L^{-1}) | Molar absorptivity(L mol^{-1}cm^{-1}) | RSD (%) | Recovery (%) | |
Proposed method | 1.5-35 | 1.79×10^{3} | 1.50 | 99.5 |
Spectrophotometric method ^{5} | 10-150 | 0.19×10^{3} | 1.11 | 98.5 |
CONCLUSION: The present spectrophotometric method describes the evaluation of NQS as analytical reagent in the development of simple, sensitive and accurate method for the determination of cephalexin in pharmaceutical formulations. Central composite design can be used to find more precise conditions for the system studied. The reagent utilized in the proposed method is cheap and readily available and the procedure does not involve any critical reaction conditions or tedious sample preparation and extraction. Statistical analysis proved that the proposed method could be applied for the analysis of cephalexin in their pure forms and in pharmaceutical formulations. Therefore, this method can be recommended for the routine analysis of cephalexin in quality control laboratories.
REFERENCES:
- O'Caiaghan SH, Kyrbi SN. In Laboratory methods in antimicrobial chemotherapy (D. S.Reeves, I. Phyllips, J. D. Williams, eds.), Churchill Livingstone, Edinburg-London-New York, 1978; 181-193.
- Bennett JV, Brodie JL, Benner E, Kirby WMM.Simplified accurate method for antibiotic assay of clinical specimens. Appl Microbiol. 1966; 14: 170–175.
- Murillo JA, Lemus JM, Garcia LF. Analysis of binary mixtures of cephalothin and cefoxitin by using first-derivative spectrophotometry. J Pharm Biomed Anal. 1996; 14: 257-266.
- Issopoulos PB, Saltas E. Analytical investigation of beta-lactam antibiotics in pharmaceutical preparations. IX. Colorimetric determination of six cephalosporins of second and third generation in the range of micromolar concentrations. Acta Pharm Hung. 1996; 66: 89-94.
- Helaleh Murad IH, Abu-Nameh Eyad SM, Jamhour Rasheed MAQ. Spectrophotometric determination of selected caphalosporins. Acta Poloniae Pharmaceutica-Drug Research. 1998; 55: 87-91.
- Barbhaiya RH, Turner P. Fluorimetric assay of cephradine, cephalexin and cephaloglycin. Br J Clin Pharmac. 1977; 4: 427-431.
- Barbhaiya RH, Turner P. Fluorimetric determination of cephalexin. P J Pharm Pharmac. 1976; 25: 791-793.
- Salto F. Separation of penicillin and cephalosporin diastereoisomers by reversed-phase high-performance liquid chromatography. JChromatogr. 1978; 161: 379-385.
- Nakagawa T, Huginaka J, Yamaoka K, Uno T. Direct high-speed liquid chromatographic determination of cephalexin in urine. JChromatogr. 1978; 147: 509-512.
- Nahata MC. High-performance liquid chromatographic determination of cephalexin in human plasma, urine and saliva. J Chromatogr. 1981; 225: 532-538.
- TingS. Reverse-phase liquid chromatographic analysis of cephalosporins. J Assoc Off Anal Chem. 1988; 71: 1123-1130.
- White ER, Laufer DN. Reversed-phase high-performance liquid chromatography of antibiotics on microbore columns. J Chromatogr. 1984; 290: 187-196.
- Signs SA, Thomas File M, James Tan S. High-pressure liquid chromatographic method for analysis of cephalosporins. Antimicrob Agents Chemother. 1984; 26: 652-655.
- Hendrix C, Thomas J, Yun LM, Hoogmartens J. Quantitative analysis of cefalexin by liquid chromatography on poly(styrene-divinylbenzene). JLiq Chromatogr. 1993; 16: 421-445.
- Hsu M.-C, Lin Y, Chung H.-C. High-performance liquid chromatographic method for potency determination of cephalexin in commercial preparations and for stability studies. J Chromatogr A. 1995; 692: 67-72.
- Pecavar A, Smidovnik A, Milivojevic D, Prosek M. A reversed phase high-performance liquid chromatographic method fordetermination of cephalexin in human plasma. J High Resol Chromatogr. 1997; 20: 674-678.
- Hendrix C, Yongxin Z, Van Houtven C, Thomas J, Roets E, Hoogrnartens J. Evaluation of analytical methods:liquid chromatography of cefalexin. Int J Pharmaceut. 1993; 100: 213-218.
- Akagawaj T, haginakak U, Amaoka I, Uno T. High speed liquid chromatographic determination of cephalexin in human plasma and urine. J Antibiotics 1978; 8: 769-775.
- Agbaba D, Eric S, Zivanov Stakic D, Vladimirov S. HPTLC assay of cephalexin andcefaclor in pharmaceuticals. Biomed Chromatogr. 1998; 12: 133-135.
- Halkar UP, Bhandari NP. Simultaneous determination of cephalexin and probenecid in pharmaceutical preparations by HPTLC.
- Coran SA, Bambagiotti-Alberti M, Giannellini V, Baldi A, Picchioni G, Paoli F. Development of a densitometric method for the determination of cephalexin as an alternative to the standard HPLC procedure. J Pharm Biomed Anal. 1998; 18: 271–274.
- Bhooshan R, Prashad V. Separation and Identification of some cephalosporin’s on impregnated TLC plates. Biomed Chromatogr. 1996; 10: 258–260.
- Jeswani RM, Sinha PK, Topagi KS, Damle MC. A validated stability indicating HPTLC method for determination of cephalexin in bulk and pharmaceutical formulation. J Pharmtech Research 2009; 1: 527-536.
- Chen LB, Wang ZF, Ferreri M, Su JL, Han B. Cephalexin residue detection in milk and beef by ELISA and colloidal gold-based one-step strip assay. J Agric Food Chem. 2009; 57: 4674-4679.
- Zhi ZL, Meyer UJ, Van den Bedem JW, Meusel M. Evalution of an automated and integrated flow-through immunoanalysis system for the rapid determination of cephalexin in raw milk. Anal Chim Acta. 2001; 442: 207-219.
- Attama AA, Nnamani PO, Agbo AN. Development of alternative assay technique for cephalexin by charge transfer interaction of the donor: Acceptor type with chloranilic acid. J Chin Pharm. 2006; 58: 11-18.
- Li QL, Chen SU. Studies on electrochemical-behavior of cephalexin. Anal Chim Acta. 1993; 282: 145-152.
- Erceg M, Kapetanovic V, Suznjevic D, Dumanovic D. Study of cephalexin and cefaclor adsorption at the mercury solution interface by ac polarography. J Microchem. 1997; 57: 73-80.
- Devi AR, Rani KS, Rao VS. Polarograpic determination of cephalosporins in pure form and in pharmaceutical preparation. Ind J Pharm Sci. 1994; 56: 64-66.
- Chen Y, Huang L, Lin Q. Rapid hydrolysis and Electrochemical Detection of Cephalexin at a Heated Glassy Carbon Electrode. Int J Electrochem Sci. 2012; 7: 7948-7959.
How to cite this article:
Irandoust M, Shariati-Rad M and Khodabakhshi M: Development of a spectrophotometric method for determination of Cephalexin using sodium 1, 2-naphthoquinone-4-sulfonate and central composite design. Int J Pharm Sci Res 2014; 5(1): 97-03.doi: 10.13040/IJPSR. 0975-8232.5(1).97-03
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97-103
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English
IJPSR
Mohsen Irandoust, Masoud Shariati-Rad* and Mina Khodabakhshi
Department of Analytical Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran
mshariati_rad@yahoo.com
14 August, 2013
13 October, 2013
26 December, 2013
http://dx.doi.org/10.13040/IJPSR.0975-8232.5(1).97-103
01 January, 2014