DEVELOPMENT OF RP-HPLC METHOD FOR SIMULTANEOUS EVALUATION OF UNIFORMITY OF DOSAGE UNITS FROM ASPIRIN AND DIPYRIDAMOLE EXTENDED-RELEASE CAPSULES
HTML Full TextDEVELOPMENT OF RP-HPLC METHOD FOR SIMULTANEOUS EVALUATION OF UNIFORMITY OF DOSAGE UNITS FROM ASPIRIN AND DIPYRIDAMOLE EXTENDED-RELEASE CAPSULES
Devi Thamizhanban * 1, Gampa Tulja Rani 2 and Kathiresan Krishnasamy 1
Department of Pharmacy 1, Annamalai University, Annamalai Nagar, Chidambaram - 608002, Tamil Nadu, India.
Malla Reddy Pharmacy College 2, Maisammaguda, Hyderabad - 500014, Telangana, India.
ABSTRACT: The aim of the present study is to develop a stability-indicating reverse-phase high-performance liquid chromatography method for the simultaneous estimation of Aspirin and Dipyridamole in combined extended-release dosage form 25/200mg, using a single unit of the capsule. Materials and Methods: Chromatographic separation was achieved with Agilent's high-performance liquid chromatography and X bridge C8 column, with the mobile phase containing a mixture of ammonium dihydrogen phosphate buffer (pH 2.5): methanol (550:450, v/v) by isocratic elution technique. The flow rate was maintained at 1.2 ml/min, and the detection wavelength was 230nm. Results: Aspirin and Dipyridamole were eluted at 4.7min and 12.6min, respectively, using the developed method. The method was linear in the range of 12.5-50μg/ml for Aspirin and 100-400 μg/ml for Dipyridamole, with an r2 value of 0.9997 and 0.9999, respectively. The sample recoveries observed were 97.63-100.96% and 97.38-98.01%, respectively, for aspirin and dipyridamole. The forced degradation studies were carried out, and the stressed samples were analyzed using the developed method. The purity angle of the peak was observed lesser than the threshold angle. Conclusion: The method could able to detect the potency of the product using one capsule. The recovery study results confirm the non-interference of formulation additives in the estimation. The purity angle from the forced degradation study confirms the non-interference from degradants in quantitating marketed formulation. Hence, the developed method is precise and accurate.
Keywords: |
Aspirin, Dipyridamole, Content uniformity, Forced degradation, RP-HPLC, stability-indicating
INTRODUCTION: Product quality is defined in terms of specifications, critical quality standards, and attributes. A critical quality attribute is a physical, chemical, biological property or characteristic that would be within an appropriate limit, range, or distribution to ensure the desired product quality 1.
Some important critical quality attributes to ensure the quality of drug products are assay, dissolution, uniformity of dosage units, and related substances.
Most of the research works were carried out in method development for assay and related substances. Developing a method for content uniformity of dosage units for a combination product is a challenging process since the drug concentrations of two drugs would be varying very high. The term “uniformity of dosage unit” is defined as the degree of uniformity in the amount of the drug substance among dosage units. The test for content uniformity of preparations presented in dosage units is based on the assay of the individual content of drug substance(s) in a number of dosage units to determine whether the individual content is within limits set. It ensures that a consistent dose of the active pharmaceutical ingredient is maintained between batches so that the patient receives the correct dose 2-3.
A stability-indicating method is an analytical procedure used to quantitate the decrease in the amount of the active pharmaceutical ingredient in drug product due to degradation 4.
The chemical name for Aspirin (ASP) is benzoic acid, 2- (acetyloxy)-, with molecular weight of 180.16 and a molecular formula of C9H8O4. White crystalline powder and odorless or has a faint odor. It is stable in dry air. In moist air, it gradually hydrolyzes to salicylic and acetic acids. Slightly soluble in water, freely soluble in alcohol, soluble in chloroform, and sparingly soluble in absolute ether. ASP is having a log P value of 1.18 and the pKa value of 3.5. 5
FIG. 1: CHEMICAL STRUCTURE (A) ASPIRIN AND (B) DIPYRIDAMOLE
The chemical name of Dipyridamole (DPM) is 2,2',2'',2'''-[(4,8-diperidinopyrimido[5,4-d] pyrimidine- 2,6- diyl) dinitrilo]- tetraethanol, with molecular weight of 504.63 and molecular formula of C24H40N8O4. Intensely yellow, crystalline powder. Very soluble in methanol, in alcohol and in chloroform, slightly soluble in water, very slightly soluble in acetone and in ethyl acetate. DPM is having the log P value of 1.5. 6
The combination of ASP and DPM is widely used to reduce thrombosis in patients with thrombotic diseases. This antithrombotic action results from additive antiplatelet effects of both drugs. ASP inhibits platelet aggregation by irreversible inhibition of platelet cyclooxygenase and thus inhibiting the generation of thromboxane A2. DPM inhibits the uptake of adenosine into platelets and endothelial cells 7.
A literature survey revealed some analytical methods were reported for the determination of ASP and DPM individually or in combination with other drugs like clopidogrel and atorvastatin using different analytical techniques like HPLC, HPTLC and LCMS from pharmaceutical formulations and other biological matrices 8-11. Few methods were also reported for the simultaneous determination of ASP and DPM using combination of liquid chromatographic and mass spectrometric detection, second-order derivative spectrophotometry, RP-HPLC method, UP-HPLC method and spectro-fluorimetric method 12-15. No methods were established for the content uniformity test. The sample size for content uniformity was a limiting factor, whereas for evaluation of assay and related substances, the sample size shall be optimized to achieve the required final concentration of drug substance for analysis. The reported methods used for evaluation of assay, where the sample size was selected from the minimum of 10 capsules.
The developed method is to evaluate the potency of the drug in drug products using a single capsule. Literature also revealed that dipyridamole is incompatible with tartaric acid, and the impact of tartaric acid in principle peak was very high. In the current study, the method was developed to elute tartaric acid at an initial time point, so that the impact on principal peaks by tartaric acid is controlled. The aim of the present study is to develop and validate a simple, accurate, and precise stability-indicating reverse phase HPLC method for the simultaneous estimation of ASP and DPM from the single extended-release capsule and by extending the run time to confirm the non-interference of excipients and degradants.
MATERIALS AND METHODS:
Chemicals and Reagents: Working standards and impurities for ASP were obtained as gift samples from Andhra sugars, for DPM was obtained from Mylan. The finished dosage form Aggrenox was procured from the pharmacy. Excipients were obtained from Signet Chemical Corporation. Ammonium dihydrogen phosphate, orthophosphoric acid, formic acid, water, and methanol of suitable HPLC and AR grade were purchased from E. Merck Co., Mumbai.
Instrumentation: The analysis was carried out using an Agilent 1200 RP-HPLC system consisting of a pump, an injector, and a photodiode array (PDA)/UV-Visible detector, with an autosampler and column heater. Data were collected and processed using Empower software. Other instruments used for analysis were Analytical Balance, Ultrasonic Bath, Centrifuge, pH meter, Oven, and Mechanical shaker. Polyvinyl difluoride filters (0.45 micron) used for sample filtration were purchased from Rankem, India.
Preparation of Mobile Phase: Preparation of buffer for mobile phase: 0.05M ammonium dihydrogen phosphate buffer was prepared by transferring 5.75 g of ammonium dihydrogen phosphate to a suitable container containing 1000 mL of water and dissolved. The pH was adjusted to 2.5 ± 0.05 using orthophosphoric acid. The solution was filtered through a 0.45 µ PVDF filter. Preparation of mobile phase: 550 mL of buffer and 450 mL of methanol were transferred into a suitable container, mixed for 5min using a stirrer, degassed through sonication.
Diluents: Three diluents were used in the analysis. Diluent-1: It was prepared by mixing 50 ml of formic acid with 950 ml of purified water, mixed for 5 min, using an overhead stirrer, degassed in a sonicator for about 10 min. Diluent-2: It was prepared by mixing 400 mL of 5% formic acid and 600 mL of methanol, using an overhead stirrer. Diluent-3: Methanol.
Preparation of Standard Solution: Preparation of ASP standard stock solution: About 25 mg of ASP was weighed accurately and transferred into a 100 ml volumetric flask, 40 ml of methanol was added and sonicated for 10 min to dissolve the material completely and 30 ml of 5% formic acid was added. The volume was made up with diluent-2 and mixed for 10 min. The resultant solution is standard stock preparation, the concentration of about 250 µg/mL of ASP.
Preparation of DPM Standard Stock Solution: About 50 mg of DPM was weighed accurately and transferred into a 50 ml volumetric flask, 20 ml of methanol was added and sonicated for 10 min to dissolve the material completely, and 15 ml of 5% formic acid was added. The volume was made up with diluent-2 and mixed for 10 min. The resultant solution is standard stock preparation, the concentration of about 1000 µg/mL of DPM.
Standard Preparation: 5 mL of ASP standard stock preparation and 10 mL of DPM standard stock preparation was transferred into a 50 mL volumetric flask. Volume was made with diluent-2 and mixed well for 10 min. The standard preparation concentration of about 25 µg/mL of ASP and 200 µg/mL of DPM.
Preparation of Test Solution: One capsule was opened and dropped the contents of the capsules into a 50 mL volumetric flask, and 20 mL of methanol was added, sonicated for about 10 min with intermittent shaking. 15 mL of 5% formic acid was added, sonicated for about 15 min with intermittent shaking, or till the capsule disperses completely. The solution was mixed using a mechanical shaker for 15 min at 200 rpm. The volume was made up with diluent-2 and mixed well. A portion of the solution was centrifuged at 3500 rpm for 10 min. 5 mL above supernatant solution was diluted to 100 mL with diluent-2 and mixed well. A portion of the above solution was filtered through a 0.45 µm PVDF membrane filter by discarding the first 4 ml of the filtrate.
Chromatographic System: HPLC analysis was performed on the Agilent HPLC system with a UV detector. Chromatographic separation of ASP and DPM was carried on X Bridge C8 column with 250 × 4.6 mm and 5 μm particle size. The isocratic condition with the mobile phase containing a mixture of buffer (pH 2.5): methanol (55:45) was programmed, and 1.2 ml/min flow rate was used for analysis, with a run time of 20 min. The detection wavelength was set at 230 nm, with the sample volume of 15µL. HPLC column was maintained at a temperature of 30 °C, and the sample was maintained at a temperature of 25 °C. The retention time was observed with 4.7 min for ASP and 12.6 min for DPM
System Suitability: Standard solutions were prepared and injected. Peak area responses for five replicate injections of the standard solutions were recorded, and system suitability was calculated. The USP tailing factor should be not more than 2.0 for ASP and DPM peak from standard preparation. The USP plate count should be not more than 1500 for ASP and DPM peak from standard preparations. The RSD of ASP and DPM peak area is NMT 2.0 from five replicate injections of standard preparations. A typical chromatogram of standard and sample were presented
Test samples were injected, and the chromatograms were recorded for the response of the analyte peak. The % content of both the drugs was calculated using the formula presented below:
Quantity of ASP present in a capsule as % of the labelled amount
% label claim for Aspirin = AT × WS × 5 × 50 × P × 100 /AS × 100 × 50 × LC × 5 × 100
AT = Peak area of ASP for Test preparation
AS = Peak area of ASP for Standard preparation
WS = Weight of ASP working standard/reference standard taken, in mg
P = Potency of ASP standard, in percent, as-is basis
L = Labelled amount of ASP in mg, per capsule
Quantity of DPM present in portion of capsule as
% of labelled amount = AT × WS × 5 × 100 × 50 × P × 100 / AS × 250 × 50 × 1 × 100 × L
AT = Peak area of DPM for Test preparation
AS = Peak area of DPM for Standard preparation
WS = Weight of DPM working standard/reference standard taken, in mg.
P = Potency of DPM standard, in percent as is basis
L = labelled amount of DPM in mg, per capsule
Calculation for the Acceptance Value:
Acceptance value (AV) = [M-X] + ks
k = Acceptability constant, for 10 units, the acceptability constant is 2.4
s = Sample standard deviation
X = mean of the individual contents (expressed as % of label claim)
M = is based on the X value.
If 98.5% ≤ X ≤ 101.5%, then M = X. if X > 101.5%, then X = 101. 5%. If X< 98.5, then M = 98.5%.
If the AV value is less than 15.0, 10 units value for content uniformity is adequate.
Analytical Method Validation: 16-17 HPLC method was validated to ensure consistent, reliable, and accurate results were obtained to determine the levels of two drugs in all the samples. The validation parameters linearity, accuracy, precision, limit of detection, the limit of quantitation, and specificity were evaluated.
Linearity: Solutions of ASP and DPM at concentration levels from about 50% to 200% of standard solution were injected into HPLC system. The linearity graph was plotted from 50% to 200%. Six injections were performed at 50% level and at 200% level and the chromatograms were recorded.
Precision: The system precision was carried out to ensure that the analytical system is working properly by injecting standard solution preparation containing ASP 25 µg/mL and DPM 200 µg/mL six times into the HPLC system as per the test procedure. The retention time and peak areas for both the drugs in all the sample solutions were measured, and % RSD was calculated. In method precision, a homogenous sample containing of ASP and DPM of a single capsule was analyzed six times, and % RSD was calculated.
Accuracy: Accuracy was performed by calculating percentage recovery by the standard addition method. A known amount of ASP and DPM were spiked with Aspirin/Extended-release dipyridamole capsules 25mg/200mg, in order to produce recovery at 50%, 100% and 150% levels of the ASP working concentration of 25 µg/mL and DPM working concentration 200 µg/mL. Spiked samples were prepared in triplicate, injected in duplicate, and the percentage recovery was calculated.
Limit of Detection (LOD): Limit of detection is the lowest concentration of the analyte that can be detected by injecting decreasing amount, not necessarily quantity by the method, under the stated experimental conditions. The minimum concentration at which the analyte can be detected is determined from the linearity curve.
The detection limit (DL) may be expressed as:
DL = 3.3 σ/S
Where, σ = the standard deviation of the response. S = the slope of the calibration curve
Limit of Quantification (LOQ): Limit of quantification is the lowest concentration of the analyte in a sample that can be estimated quantitatively by injecting a decreasing amount of drug with acceptable precision and accuracy under the stated experimental conditions of the method. The limit of quantitation can be obtained from the linearity curve. The detection limit (DL) may be expressed as:
DL = 10 σ/S
Where, σ = the standard deviation of the response. S = the slope of the calibration curve
Specificity: Blank, standard, placebo, sample solutions, and impurity were prepared and injected into the chromatographic system for identification and interference of the ASP and DPM Peak.
Solution Stability: The standard and sample solutions were prepared and injected. Replicate injections of the standard and sample solutions were made at the following time intervals at 5 °C: Initial, 24 h, and 48 h. The concentration of standard at 24 h and 48 h were compared to that of the initial.
Forced Degradation Studies (Stress Testing): In order to develop a stability-indicating method for estimation of ASP and DPM, stress studies were carried out and validated the stability-indicating property of the proposed method. The chromatograms of the stressed samples were evaluated for peak purity of ASP and DPM peak using Empower networking software
Acid Degradation Studies: Weighed and transferred five capsules of Aspirin/Extended-Release Dipyridamole Capsules into a 250 mL volumetric flask. Added about 20 ml of methanol, sonicated for about 10 min with intermittent shaking. Added 15 mL of 5% formic acid, sonicated for about 15 min with intermittent shaking till the capsules disperse completely. Shaken on a mechanical shaker for 15 min at 200 rpm. Diluted the volume with 5mL of 1N methanolic HCl. Benchtop kept for 1.5 h. Neutralized with 5 mL of IN methanolic NaOH,
Base Degradation Studies: Weighed and transferred five capsules of Aspirin/Extended-Release Dipyridamole Capsules into a 250 mL volumetric flask. Added about 20 ml of methanol, sonicated for about 10 min with intermittent shaking. 15 mL of 5% formic acid was added and sonicated for about 15 min with intermittent shaking till the capsules disperse completely. Shaken on a mechanical shaker for 15 min at 200 rpm. Diluted the volume with 5mL of IN Methanolic NaOH. Benchtop kept 1.0 h neutralized with 5 mL of IN Methanolic HCl.
Oxidation Stress Studies: Weighed and transferred five capsules of Aspirin/Extended-Release Dipyridamole Capsules into a 250 mL volumetric flask and added about 20 ml of methanol, sonicated for about 10 min with intermittent shaking. Added 15 mL of 5% formic acid, sonicated for about 15 min with intermittent shaking till the capsules disperse completely. They were shaken on a mechanical shaker for 15 min at 200 rpm. Diluted the volume with 5 mL of 30% hydrogen peroxide and heated on a water bath at 60 °C for 15 min.
Photolytic Degradation Studies: Weighed and transferred five capsules of Aspirin/Extended-Release Dipyridamole Capsules (stressed under UV light for 24 h) into a 250 mL volumetric flask. Added about 100 ml of methanol, sonicated for about 10 min with intermittent shaking.
Thermal Degradation Studies: Weighed and transferred five capsules of Aspirin/Extended Release Dipyridamole Capsules (Heated at 105°C in an oven for 1.5 h) into a 250 mL volumetric flask. Added about 100 ml of methanol, sonicated for about 10 min with intermittent shaking.
Filter Study: A sample was prepared as per the method for the filter study. This sample was divided into three portions. One portion of the prepared sample was centrifuged at 3500 RPM for 10 min. The centrifuged sample was used as a control for the filter study. The second portion of sample was filtered through 0.45µ PVDF filter, and the filtrate was collected after discarding the first 4 mL, 5 mL, 6 mL, and 7 mL of the filtrate. The third portion of sample was filtered through 0.45 µ nylon filter and the filtrate was collected after discarding the first 4 mL, 5 mL, 6 mL and 7 mL of the filtrate. The centrifuged and filtered samples were injected.
Robustness: Standard solution was prepared and injected into the chromatographic system as per the conditions specified in the method. The same standard solution was re-injected by changing one parameter at a time, keeping other parameters constant. Method Parameters:
- Flow Rate (Normal -1.2 mL/min), a. Flow minus ~ 1.1 mL/min,b. Flow plus ~ 1.3 mL/min.
- Column Operating Temperature (Normal temperature is 30 °C), a. Temperature minus ~ 25 °C, b. Temperature plus ~ 35 °C.
- Buffer pH variation (Normal Buffer pH 2.5), a. pH minus ~ pH 2.3 b. pH plus ~ pH 2.7.
- Mobile Phase Composition Variation (Normal Composition is Buffer: Methanol, 55:45) a. MPVl ~ Buffer: Methanol (57:43); b. MPV2 ~ Buffer: Methanol (53:47) c. MPV3 ~ Buffer: Methanol (56:44); d. MPV4 ~ Buffer: Methanol (54:46)
RESULTS AND DISCUSSION: The initial screening for diluents was performed based on the chemical nature of the molecule. Since ASP is sensitive to basic conditions, diluents selected are of acidic nature with lower pH values. Chromatographic parameters were preliminary optimized to develop a stability-indicating method for ASP and DPM with a short analysis time (20 min). To separate the degradants from main analyte, isocratic system was developed to elute the impurities, thus capturing all the possible degradants of both the components. All degradants shall be separated using with increased column length. Hence, a longer column (250 × 4.6 mm, 5 μm particle size) was selected to have a shortest possible runtime without compromising on the resolution. In order to identify a suitable organic modifier, various organic solvents like acetonitrile and methanol were tested. Methanol produced better selectivity with low column back pressures. Ammonium dihydrogen phosphate buffer gave sharp peaks for both the components compared to other buffers. Diluents selected for the preparation standard and sample solutions were based on the extraction and stability of both the drugs.
The system suitability was performed by injecting 15µL combined standard preparation into the chromatographic system for five times, the chromatograms were recorded, and responses were measured for the ASP and DPM peaks. The system suitability parameters summary is presented in Table 1 and the typical chromatogram for standard is presented in Fig. 2a. The % RSD for peak area of five replicate injections was observed with 0.11% for ASP and 0.07% for DPM. The marketed product Aggrenox 25/200mg was analysed using the developed method and results were presented in Table 2. The sample chromatogram was presented in Fig. 2b. The acceptance value is meeting the acceptance criteria of below 15.
TABLE 1: SYSTEM SUITABILITY FOR ASPIRIN AND DIPYRIDAMOLE
Parameters | Drug | Mean ± SD | % RSD |
Retention time (Rt) | ASP | 4.42 ± 0.052 | 1.19 |
DPM | 12.67 ± 0.059 | 0.46 | |
Peak area | ASP | 802848 ± 867 | 0.11 |
DPM | 7253432 ± 4893 | 0.07 | |
Tailing factor(T) | ASP | 1.15 ± 0.008 | 0.73 |
DPM | 1.50 ± 0.016 | 1.09 | |
Theoretical plates (N) | ASP | 7974 ± 48 | 0.60 |
DPM | 6738 ± 82 | 1.22 |
TABLE 2: CONTENT UNIFORMITY FOR ASPIRIN AND DIPYRIDAMOLE EXTENDED RELEASE CAPSULES
S. no. | Content uniformity (BH30472) | |
ASP | DPM | |
1 | 100.2 | 99.8 |
2 | 99.8 | 100.1 |
3 | 100.3 | 99.5 |
4 | 100.2 | 98.9 |
5 | 100.5 | 101.2 |
6 | 99.7 | 101.1 |
7 | 99.5 | 100.5 |
8 | 100.1 | 99.8 |
9 | 100.5 | 98.9 |
10 | 100.3 | 100.3 |
Average (X) | 100.11 | 100.01 |
S | 0.34 | 0.80 |
% RSD | 0.34 | 0.80 |
K | 2.4 | 2.4 |
M | 100.11 | 100.01 |
AV | 0.81 | 1.92 |
FIG. 2: A TYPICAL CHROMATOGRAM OF (A) STANDARD FOR ASPIRIN AND DIPYRIDAMOLE, AND (B) SAMPLE FOR ASPIRIN AND DIPYRIDAMOLE EXTENDED-RELEASE CAPSULE 25/200mg
The linearity of the method was established by injecting solutions of ASP and DPM. The data is shown in Tables 3 and 4. The linearity plot of ASP and DPM was presented in Fig. 3 and 4. Correlation coefficient square (r2) for ASP and DPM met the acceptance criteria of more than 0.997. RSD of peak area at 50% and 200% is less than 2.0%. The linear regression data shows that the method is linear over the entire concentration range (50% to 200% of the standard concentration 25 µg/mL of ASP and 200 µg/mL of DPM), and it is adequate for its intended concentration range. The LOD values for ASP and DPM were determined to be 0.56 µg/ml and 3.95 µg/ml, and the LOQ values were 1.69µg/ml and 11.96 µg/ml, respectively.
TABLE 3: LINEARITY STUDY FOR ASPIRIN AND DIPYRIDAMOLE
S. no. | Aspirin | Dipyridamole | ||
Conc. (µg/ml) | Peak
Area |
Conc. (µg/ml) | Peak
Area |
|
1 | 12.5 | 389955 | 100 | 3665260 |
2 | 20 | 620314 | 160 | 5839184 |
3 | 25 | 772524 | 200 | 7265677 |
4 | 30 | 935915 | 240 | 8815845 |
5 | 38 | 1171020 | 300 | 11033348 |
6 | 50 | 1553957 | 400 | 14614871 |
FIG. 3: LINEARITY OF ASPIRIN
FIG. 4: LINEARITY OF DIPYRIDAMOLE
TABLE 4: VALIDATION PARAMETERS ESTABLISHED BY LINEARITY AND PRECISION
Parameters | ASP | DPM |
Linearity (µg/ml) | 12.5-50 | 100 -400 |
correlation co-efficient (r2) | 0.9997 | 0.9999 |
Regression equation | y = 30991x + 794.31 | y = 36634x - 8907.2 |
Method precision (% RSD) | 1.02 | 0.57 |
System precision (at 50% level) (% RSD) | 0.18 | 0.27 |
System precision (at 200% level) (% RSD) | 0.18 | 0.13 |
LOD (µg/ml) | 0.56 | 3.95 |
LOQ (µg/ml) | 1.69 | 11.96 |
The precision of the test method was evaluated by repeatability studies by evaluating ten test samples of ASP and DPM extended-release capsules 25mg/200mg. The % relative standard deviation of ASP and DPM is presented in Table 4, which was observed within the acceptance criteria limit of not more than 15% according to the ICH guideline.
The recovery experiments were performed by adding a known quantity of pure standard drug into the solution of the capsule. The sample was spiked with the standard at levels 50%, 100%, and 150% of test concentration were evaluated for content uniformity in triplicate, which was observed with the % RSD less than 2%, and the results are presented in Table 5.
TABLE 5: ACCURACY (RESULTS OF RECOVERY STUDY)
Level of Recovery (%) | Conc. actual (µg/ml) | Conc. Added (µg/ml) | Mean % recovery ± SD (n=3) | % RSD | ||||
ASP | DPM | ASP | DPM | ASP | DPM | ASP | DPM | |
50 | 25 | 200 | 12.5 | 100 | 100.8 ± 0.20 | 97.68 ± 0.30 | 0.20 | 0.30 |
100 | 25 | 200 | 25 | 200 | 100.61± 0.26 | 97.66 ± 0.12 | 0.26 | 0.12 |
150 | 25 | 200 | 37.5 | 300 | 97.74 ± 0.10 | 97.94 ± 0.07 | 0.10 | 0.07 |
Placebo Interference: Chromatograms of placebo showed no peaks at the retention times of ASP and DPM peaks. This indicates that the excipients used in the formulation do not interfere in the estimation of ASP and DPM in capsules. The placebo chromatogram is shown in Fig. 5a.
Impurity Interference: The chromatogram recorded by spiking the standard preparation with all impurities in the concentration of 0.3% of test preparation was found that all the impurities are separated from the main analyte ASP and DPM. Chromatogram of impurity interference is shown in Fig. 5b. The specificity data is presented in Table 6.
TABLE 6: SPECIFICITY (INTERFERENCE OF BLANK, PLACEBO AND IMPURITIES)
Sample Name | Retention Time (min) | Interference |
Salicylic acid | 6.486 | Nil |
Dipyridamole Impurity-B | 3.438 | Nil |
Dipyridamole Impurity-F | 3.947 | Nil |
Dipyridamole Impurity-D | 15.158 | Nil |
Dipyridamole Impurity-E | 26.25 | Nil |
Dipyridamole Impurity-C | ND | Nil |
Dipyridamole Impurity-A | ND | Nil |
Spiked sample Aspirin | 4.639 | Nil |
Spiked sample Dipyridamole | 13.06 | Nil |
Blank | ND | Nil |
FIG. 5: A TYPICAL CHROMATOGRAM OF (A) PLACEBO FOR INTERFERENCE AND (B) IMPURITY INTERFERENCE
The solution stability study reveals the concentration of the 48 h injections of standard solution differed by less than 2.0%, and 48 h of sample solution differed by less than 2.0% when compared to the initial sample solution. Hence, the standard and sample solutions can be used up to 48 h after its preparation if it is stored at 5 °C. The solution stability results are presented in Table 7.
TABLE 7: SOLUTION STABILITY OF ASPIRIN AND DIPYRIDAMOLE
Time (hrs) | Standard conc. (µg/ml) | Difference from initial (%) | Sample Assay (%) | Difference from initial (%) | ||||
ASP | DPM | ASP | DPM | ASP | DPM | ASP | DPM | |
Initial | 25.409 | 199.399 | N/A | N/A | 101.34 | 100.54 | N/A | N/A |
24 h | 25.296 | 198.814 | 0.44 | 0.29 | 101.47 | 100.35 | 0.13 | 0.19 |
48 h | 25.221 | 198.69 | 0.74 | 0.36 | 101.66 | 100.14 | 0.32 | 0.40 |
Forced Degradation Studies (Stress Testing): From the forced degradation sample chromato-grams, all degradants peaks were resolved from ASP and DPM peak in the chromatograms of all samples. For all forced degradation samples, the purity angle found to be less than the threshold angle, which indicates that there is no interference from degradants in quantitating the ASP and DPM in capsules. The percentage drug content after forced degradation, purity threshold, and purity angle was performed for all the stressed samples, and unstressed samples were presented in Table 8, and chromatogram were presented in Fig. 6.
TABLE 8: ASSAY AND PEAK PURITY OF FORCED DEGRADATION STUDIES
Stress study | Aspirin | Dipyridamole | ||||
%
Assay |
Purity Angle | Purity threshold | %
Assay |
Purity Angle | Purity threshold | |
Control Sample | 100.3 | 0.105 | 0.7 | 100.25 | 0.019 | 0.3 |
Acid stress | 88.75 | 0.138 | 0.363 | 99.98 | 0.019 | 0.244 |
Base stress | 86.35 | 0.152 | 0.857 | 83.15 | 0.018 | 0.322 |
peroxide stress | 75.73 | 0.157 | 0.599 | 99.95 | 0.023 | 0.281 |
UV stress | 100.2 | 0.109 | 0.798 | 100.56 | 0.025 | 0.323 |
Heat stress | 83.76 | 0.153 | 0.475 | 100.95 | 0.021 | 0.275 |
FIG. 6: CHROMATOGRAM OF ASPIRIN AND DIPYRIDAMOLE SAMPLE (A) UNSTRESSED (B) ACID STRESSED, (C) BASE STRESSED, (D) OXIDATION STRESSED, (E) HEAT STRESSED AND (F) UV LIGHT STRESSED SAMPLES
Filter Study: The area found in the filtered fractions of the sample solution was comparable to the area found in the centrifuged portion of the sample solution. There is no significant difference in the area between different volumes filtered. Therefore, the filters are suitable for use, and the discarding of 4 mL of sample solution as filtrate, as stated in the method, is a suitable volume to discard before collecting for analysis by HPLC. The results are presented in Table 9.
TABLE 9: FILTER STUDY WITH PVDF AND NYLON FILTERS
Sample Name | % Difference of Assay from unfiltered sample | |
ASP | DPM | |
Centrifuged Sample (10 min @, 3500rpm) | N/A | N/A |
0.45µ PVDF filtrate sample, 4 mL discarded | 0.04 | 0.03 |
0.45µ PVDF filtrate sample, 5 mL discarded | 0.21 | 0.12 |
0.45µ PVDF filtrate sample, 6 mL discarded | 0.19 | 0.25 |
0.45µ PVDF filtrate sample, 7 mL discarded | 0.15 | 0.14 |
0.45µ Nylon filtrate sample, 4 mL discarded | 0.49 | 0.34 |
0.45µ Nylon filtrate sample, 5 mL discarded | 0.29 | 0.18 |
0.45µ Nylon filtrate sample, 6 mL discarded | 0.28 | 0.15 |
0.45µ Nylon filtrate sample, 7 mL discarded | 0.52 | 0.62 |
Robustness: No significant change was observed in retention time after individually changing the conditions of the flow rate of mobile phase by ±0.1mL/min, column operating temperature by ±5 °C, pH of the buffer by ±0.2 units and mobile phase composition variation by ±1% absolute. However, a significant difference in retention time observed while varying the mobile phase composition by ±2% absolute.
Calculations for all other system suitability parameters met the acceptance criteria, and the data generated are comparable with the normal conditions. Based on the above result, it is concluded that the method is unaffected by small, deliberate variations in flow rate, column temperature, pH of buffer, and mobile phase composition variation. The results are presented in Table 10.
TABLE 10: ROBUSTNESS STUDY- COMPARISON OF SYSTEM SUITABILITY AND RETENTION TIME
Parameters | Condition | Retention time | Peak area (mean ± SD) (n=5) |
USP tailing factor | USP plate count | ||||
ASP | DPM | ASP | DPM | ASP | DPM | ASP | DPM | ||
Normal Condition | (Buffer pH 2.5: Methanol (55:45), 1.2mL/min, 30°C) | 4.79 | 12.670 | 851115
±0.10 |
7259212
±0.23 |
1.43 | 1.79 | 8113 | 5800 |
Flow Rate Minus | 1.1 mL/min | 5.22 | 15.148 | 930245 ±0.20 | 7907613 ±0.69 | 1.44 | 1.79 | 8300 | 5672 |
Flow Rate Plus | 1.3 mL/min | 4.42 | 12.965 | 790440 ±0.40 | 6788328 ±0.57 | 1.42 | 1.78 | 7558 | 5404 |
pH | 2.3 | 4.80 | 13.245 | 859316 ±0.39 | 7449066 ±0.25 | 1.51 | 1.77 | 7230 | 5516 |
pH | 2.7 | 4.68 | 13.536 | 836791 ±0.16 | 7508209 ±0.22 | 1.52 | 1.72 | 7173 | 5789 |
Column Temperature |
25°C | 5.05 | 16.009 | 859807 ±1.34 | 7324933 ±0.24 | 1.39 | 1.72 | 7045 | 5089 |
Column Temperature |
35°C | 4.55 | 12.377 | 856848 ±0.28 | 7378215 ±0.13 | 1.43 | 1.77 | 8467 | 6353 |
MPV1 | Buffer: Methanol, (57:43) |
5.17 | 18.421 | 846392 ±0.08 | 7507376 ±0.12 | 1.5 | 1.83 | 7528 | 5746 |
MPV2 | Buffer: Methanol, (53:47) |
4.41 | 10.682 | 848255 ±0.28 | 7382697 ±0.12 | 1.42 | 1.72 | 7775 | 5648 |
MPV3 | Buffer: Methanol, (56:44) |
4.97 | 15.313 | 849761 ±0.25 | 7352812 ±0.16 | 1.46 | 1.77 | 7520 | 5549 |
MPV4 | Buffer: Methanol, (54:46) |
4.62 | 11.878 | 847599 ±0.07 | 7333893 ±0.06 | 1.46 | 1.7 | 7383 | 5476 |
CONCLUSION: Novelty in this research work involves establishing better isolation and elution of active ingredients and degradants in a short run time of 20 min, using 2 diluents, and mobile phase at lower pH of around 2, which resulted in longer solution stability and better resolution. The developed method was capable of eluting degradation products. The drug peaks observed from chromatograms were not interfered by degradants and formulation additives. The method was validated in compliance with the ICH guidelines. Hence, this developed method can be conveniently adopted for routine quality control analysis of content uniformity of ASP and DPM extended release capsules.
ACKNOWLEDGEMENT: The authors are thankful to Malla Reddy Pharmacy College for providing necessary facilities and also gratitude towards Andhra sugars and Mylan laboratories, for providing gift sample.
CONFLICTS OF INTEREST: The author(s) declare(s) that there is no conflict of interest’.
REFERENCES:
- International Conference on Harmonization International Conference on Harmonisation of technical requirements for registration of pharmaceuticals For Human Use. Harmonized Tripartite Guideline. Pharmaceutical Development Q8 (R2), version-4, August 2009.
- Brown WE: Uniformity of Dosage Units. United sates pharmacopoeia 32–National formulary 27: 382.
- Guidance for Industry. Q4B Evaluation and Recommendation of Pharmacopoeial Texts for Use in the ICH Regions. Annex 6, Uniformity of Dosage Units General Chapter, Revision 1. June 2014.
- Blessy MN, Patel RD, Prajapati PN and Agrawal YK: Development of forced degradation and stability indicating studies of drugs-A review. JPA 2014; 4(3): 159-65.
- Aspirin USP, United States pharmacopoeia 32–National formulary 27: 1582.
- Dipyridamole USP: United States Pharmacopoeia 32–National formulary 27: 2181.
- AGGRENOX® (aspirin/extended-release dipyridamole) capsules, for oral use, Pack insert leaflet, Boehringer Ingelheim Pharmaceuticals, Inc. Ridgefield, CT 06877 USA, Revised: November 2018.
- Zhang J, Miller RB and Jacobus R: Development and validation of a stability- indicating HPLC method for the determination of degradation products in dipyridamole injection. Chromatographia 1997; 44(5/6).
- Pulgarin JAM, Molina AA and Lopez PF: Direct determination of DPMyridamole in serum. Analytical Biochemistry 1997; 245(1): 8-16.
- Kachhadia PK, Doshi AS and Joshi HS: Validated column high-performance liquid chromatographic method for determination of aspirin and clopidogrel in combined tablets in the presence of degradation products formed under ICH recommended stress conditions. Journal of AOAC International 2009; 92(1): 152-57.
- Rajput SJ, George RK and Ruikar DB: Chemometric Simulataneous estimation of clopidogrel bisulphate and aspirin from combined dosage form. Indian Journal of Pharmaceutical Sciences 2008; 70(4): 450-54.
- Umapathi P: Determination of atenolol, nifedipine, aspirin and dipyridamole in tablet preparations by second-order derivative spectrophometry. International Journal of Pharmaceutics 1998; 108(1): 11-19.
- Prakash K, Kalakuntla RR and Sama JR: Rapid and simultaneous determination of aspirin and dipyridamole in pharmaceutical formulations by reversed-phase high performance liquid chromatography (RP-HPLC) method. African Journal of Pharmacy and Pharmacology 2011; 5(2): 244-51.
- Rajput AP, Manohar C and Sonanis: Development and validation of a rapid RP-UPLC method for the determination of aspirin and dipyridamole in combined capsule formulation. International Journal of Pharmacy and Pharmaceutical Sciences 2011; 3(2): 156-60.
- Hammud HH, El-Yazbi FA, Mahrous ME, Sonji GM and Sonji NM: Stability-Indicating Spectrofluorimetric and RP-HPLC Methods for the Determination of Aspirin and Dipyridamole in their Combination. The Open Spectroscopy Journal 2008; 2: 19-28.
- Ermer J: Analytical Validation within the Pharmaceutical Environment. Method validation in pharmaceutical analysis: a guide to best practice. 2nd edition, chapter-1, 2005; WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim 3-20.
- International Conference on Harmonization International Conference on Harmonisation of technical requirements for registration of pharmaceuticals For Human Use, Harmonized Tripartite Guideline, Q2 (R1) Validation of analytical procedures : text and methodology 2005.
How to cite this article:
Thamizhanban D, Rani GT and Krishnasamy K: Development of RP-HPLC method for simultaneous evaluation of uniformity of dosage units from aspirin and dipyridamole extended-release capsules. Int J Pharm Sci & Res 2020; 11(5): 2470-81. doi: 10.13040/IJPSR.0975-8232.11(5).2470-81.
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Article Information
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2470-2481
780
858
English
IJPSR
D. Thamizhanban *, G. T. Rani and K. Krishnasamy
Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, India.
devrajmphd@gmail.com
01 February 2020
26 March 2020
28 March 2020
10.13040/IJPSR.0975-8232.11(5).2470-81
01 May 2020