EFFECT OF CALCIUM PHOSPHATE COMPOSITE WITH DOXYCYCLINE AS A GRAFT FOR BONE DEFECT

The objective was to determine the effect of calcium phosphate composite with doxycycline molecularly included as a graft on bone defect after tooth extraction for slow delivery of drug. Methods: animal were distributed in to four groups: Group 1 (Blood Clot), Group 2 (TCP), Group 3 (TCP/PCL/PLGA), and Group 4 (TCP/PCL/ PLGA/ DOX/CD). An alveolar bone defect was created, and the analysis was conducted at preset intervals of T1 (14 days) and T2 (28 days). Results: The results of the histological study using H&E staining demonstrated the existence of newly created bone in all experimental groups. Group 4 exhibited the biggest area of newly formed bone at T1, and this observation was consistent across all time points examined. Group 4 exhibited a consistent volume across the measured time intervals, as evidenced by the existence of more advanced and structured trabeculated bone in T2 in comparison to T1. Even more Group 4, notably T2, displayed the highest proportion (47%) of newly formed bone area in comparison to Group 1 (36%), Group 2 (33%), and Group 3 (31%). The statistical analysis indicated that there were not statistically significant differences at recorded times both within the same groups and between the different groups (p>0.05). The number of osteoclasts observed in Group 4 of T2 exhibited a reduction compared to Group 4 of T1, suggesting that DOX has an inhibitory effect on the formation of osteoclasts. Therefore, the doxycycline/CD combination promotes the production of new bone during the crucial early phases of bone repair (T1).