EFFECT OF DICHLOROMETHANE EXTRACT OF ALSTONIA SCHOLARIS LEAVES IN DIFFERENT MODELS OF DIABETIC NEPHROPATHY IN RATS

Aim: The main of the study is to study the Effect of Various Polar & Non-Polar Extracts of Alstonia scholaris leaves in Different Models of Diabetic Nephropathy in Rats. Material & Methods: Leaves of selected plant was extracted by using various solvents, i.e., Pet ether, Dichloromethane, Ethyl acetate, methanol and water. All the extracts were screened for the presence of various active phytocompounds i.e., alkaloids, terpenoids, tannins, flavonoids and phenolic compounds etc. For the pharmacological studies, OECD guidelines were used for the toxicity studies, and 1/10^th and 1/5^th doses used for the entire study. All the extracts were evaluated by different diabetic nephropathy model. After an assessment of activity, various biochemical parameters were measured. Result: There was a significantly high level of blood urea nitrogen in diabetic nephropathy animals compared to the normal animals; 28 days treatment regimen showed a significant reduction in elevated urea level. Elevated serum creatinine level was observed in diabetic animals which were significantly reduced by treatment groups; the highest reduction was observed in extracts group treated. Elevated serum creatinine level was observed in diabetic control, nephropathy control and diabetic nephropathy control groups; after the treatment with the drugs, the creatinine level was significantly reduced. Urine protein and albumin excretion were highly increased in the diseased group (DC, NC, and DNC group). After the treatment regimen, the group showed a significant effect on urinary excretion profile. Conclusion: The drugs controlling both metabolic and hemodynamic processes can effectively revert the progressive diabetic nephropathy in diabetes patients. Dichloromethane extract ameliorates diabetic nephropathy in both the models better than any other treatment regimen studied.