ELICITATION, BIOCONVERSION AND QUANTIFICATION OF BERBERINE FROM CISSAMPELOS PARIERA CALLUS CULTURES
AbstractCissampelos pariera, an important medicinal plant was subjected to elicitation and bioconversion of precursors for the formation of Berberine, an important medicinal component. In vitro cultures of Cissampelos pariera, was successfully established using stem and leaf explants. MS Media fortified with IAA (2 mg/l) alone and MS media enriched with IAA (2mg/l) showed profuse callusing from leaf and stem explants respectively. Elicitation with yeast resulted in the highest percentage of berberine yield from leaf callus (0.055%). Elicitation with Bacillus subtilis showed the presence of 0.0294% berberine in leaf callus. Elicitation with Aspergillus niger showed the highest yield of 0.0019% from stem callus. Addition of precursor components Tryptophan and Phenylalanine did not increased the berberine from leaf callus. But addition of precursor components Tryptophan and Phenylalanine showed the bioconversion to 0.0019% and 0.0022% of berberine in stem callus. The study reports a standardized protocol for the efficient conversion of precursors to breberine and also to elicit the production using elicitors
Download