ESTIMATION OF TEICOPLANIN IN PARENTERALS BY RP-HPLCAbstract
A simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation of Teicoplanin in Parenteral dosage form. An Xterra RP C18, 250×4.6 mm, column with 5 µm particle size and the mobile phase consisting of 0.02M Potassium Dihydrogen Orthophosphate + 0.02M Dipotassium Hydrogen Orthophosphate + 0.5% Triethyl Amine in water pH: 3.2 adjusted with Orthophosphoric Acid: Acetonitrile (40:50). Acetonitrile in the isocratic mode was used. The flow rate was 0.5 ml/min and the effluents were monitored at 210 nm. The retention time was 4.178 min. The detector response was linear in the concentration of 24.1-289.2 mcg/mL for Teicoplanin, the respective linear regression equation being Y (-242937.9235) = 71026.949x + 174544.8363 for Teicoplanin. The Limit of Detection (LOD) and The Limit of Quantification (LOQ) were 1.205 mcg/mL and 3.615 mcg/mL respectively for Teicoplanin. The percentage assay of Teicoplanin was 99.64 %. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Teicoplanin in bulk drug and in its pharmaceutical dosage forms.
Ravi Pratap Pulla, B. S. Sastry , Y. Rajendra Prasad and N. Appala Raju
Department of Pharmaceutical Chemistry, SSJ College of Pharmacy, V.N.Pally, Gandipet, Hyderabad-500 075, Andhra Pradesh, India
13 April, 2011
21 May, 2011
26 June, 2011
01 July, 2011