ETHNOMEDICINAL VALUES AND ANTIDIABETIC POTENTIAL OF CLERODENDRUM SPP. OCCURRING IN NORTHEASTERN REGION
HTML Full TextETHNOMEDICINAL VALUES AND ANTIDIABETIC POTENTIAL OF CLERODENDRUM SPP. OCCURRING IN NORTHEASTERN REGION
Janmoni Kalita * 1, S. Sureshkumar Singh 2 and Mohamed Latif Khan 3
Department of Biotechnology 1, Gauhati University, Guwahati - 781014, Assam, India.
Department of Forestry 2, North Eastern Regional Institute of Science and Technology (Deemed University), Nirjuli - 791109, Arunachal Pradesh, India.
Department of Botany 3, Dr. Harisingh Gour Central University, Sagar - 470003, Madhya Pradesh, India.
ABSTRACT: The present study was undertaken to systematically analyze, document the traditional knowledge of Clerodendrum species use for the treatment of various human ailments from NER. The information was collected by literature survey as well as by consulting questionnaire with the villagers and local communities of NER. A total of twelve Clerodendrum species were collected, and among them, C. colebrookianum, C. indicum, C. viscosum were found to have multi-medicinal values that were widely used for the treatment of various diseases. Further antidiabetic properties of Clerodendrum species were evaluated by α-amylase and α-glucosidase assay. The result showed 4 species, C. indicum, C. japonicum, C. serratum, C. viscosum exhibited significant (>50%) α-amylase inhibition properties while 3 other species, C. colebrookianum, C. inerme, C. viscosum displayed significant α-glucosidase inhibition properties. Lowest IC50 values were observed in C. serratum of α-amylase (45µg/ml) and C. viscosum of α-glucosidase (47 µg/ml). A detailed scientific investigation of the biochemical compounds and metabolites with potential biological activities in Clerodendrum species may lead to the discovery of potential drug candidates against life-threatening diseases and will help in scientific understanding and proper utilization in traditional and modern health care systems of the country in particular and the world in general.
Keywords: |
Clerodendrum, Diversity, Ethnobotany, Tribal communities, Northeastern region
INTRODUCTION: Clerodendrum is a very large and medicinally important genus reported to have more than 400 species distributed in tropical and subtropical regions of the world 1. There are about 23 species being reported in India where 16 species and 1 variety are reported from the state of Arunachal Pradesh alone 2.
These plants are found abundantly growing in northeastern region (NER) and widely used continuously by the local peoples for treatment of many diseases such as anti-microbial, anti-helmintic, anti-inflammatory, anti-malarial, anti-diabetic, hepatoprotective, indigestion, high blood pressure, high fever, asthma, etc. Phytochemical study of Clerodendrum species reported to have major compounds of alkaloids, phenolics, flavonoids, steroids etc. and various biological activities like antibacterial, antimicrobial, insecti-cidal, antihypertensive, antioxidant etc. have been reported 1.
Type II diabetes (non-insulin-dependent diabetes mellitus) is a chronic endocrine disorder characterized by hyperglycemia in which blood sugar levels are elevated either because the pancreas do not produce enough insulin or cells do not respond to the produced insulin 3. In the recent past, in-vitro screening of herbal-based inhibitors for alpha (α-) amylase and α-glucosidase enzymes have been important approaches to researchers for the discovery of antidiabetic drugs. α-amylase is the enzyme that hydrolyses the polysaccharide (starch) to oligosaccharides (maltose), and α-glucosidase catalyzes the final step to release the absorbable glucose. Hence, the inhibition of these enzymes leads to a decrease in blood glucose level, which plays an important role in the management of diabetic complications4. Since these enzymes play key roles in digestion and intestinal absorption of sugar in the diet, their inhibitors are the potential targets in the development of lead compounds for the treatment of diabetes 5. In-vitro screening of α-amylase and α-glucosidase enzyme inhibitors have been reported from many plants including some species of Clerodendrum such as C. bungei, C. multiflorum and C. volubile 6, 7, 8 and other plants namely, Artocarpus altilis, Artocarpus heterophyllus, Berberis aristata, Cassia auriculata, Cinnamomum zeylanicum, Piper betel, Terminalia arjuna etc.9, 10
Therefore, the present investigation was carried out to document on the ethnobotany of North East Clerodendrum species and evaluation of an antidiabetic property by in-vitro α-amylase and α -glucosidase assay.
MATERIALS AND METHODS:
Study Area, Species Identification and Herbarium Preparation: Field surveys (2011-2015) were conducted in six states of NER (Arunachal Pradesh, Assam, Manipur, Meghalaya, Nagaland, Mizoram) and Clerodendrum species were collected, identified by examining the morphological and reproductive features with consulting various available floras 11, 12, Botanical Survey of India, Itanagar and available identifying keys 13, 2. The plant list (http://theplantlist.org) was used for the valid names. Each of the herbarium was given a specific voucher number and was deposited in the Herbarium of Department of Forestry, NERIST, Nirjuli, Arunachal Pradesh.
Field Survey and Ethnobotany of Clerodendrum Species from NER: All peer-reviewed journals, book chapters, particularly on ethno-medicinal uses of Clerodendrum species from NER,, were selected for this review and collected by searching major scientific electronic databases including Google Scholar, PubMed, Science Direct, etc.
In terms of journal contribution to this review, the Journal of Traditional Knowledge (13nos.) and Journal of Economic and Taxonomic Botany (11nos.) provided the majority of articles carrying information on the ethnobotany of Clerodendrum species from NER. Further validation was done by making field trips to village areas of tribal communities, and information on medicinal uses of Clerodendrum species was collected through modified semi-structured questionnaire 14 (Appendix 1). A total of 100 informants were selected at random during house-to-house surveys. The knowledge of medicinal plants >30 years of age was taken into consideration.
Preparation of Plant Material for Bioassay: A total of the twelve Clerodendrum species were collected during the field survey. However, only seven (7) species viz. C. colebrookianum (CC), C. inerme (CIN), C. indicum (CI), C. japonicum (CJ), C. philippinum (CP), C. serratum (CS) and C. viscosum (CV) were selected for antidiabetic assay based on their medicinal values and availability of plant samples in wild habitats. Young, tender, and disease-free leaves of selected seven Clerodendrum species were rinsed with tap water followed by drying in a hot-air oven (60 °C) for 3-12 h, grounded into powder by using a kitchen grinder.
Preparation of Crude Methanol Leaf Extract: Dry powdered leaves (1kg) of seven Clerodendrum species were suspended in methanol (ME) and kept overnight in a rotary shaker (Scigenics Biotech, Chennai) at 100 rpm. The slurry was filtered on sterile Whatman filter paper (110mm), and the green-colored filtrate was vacuum dried (below 25 °C) in a rotary evaporator (RV-10, IKA, Germany). The yield of the extracts was recorded, and the samples were finally stored at -20º separately. The crude methanol extracts were screened for their inhibitory activities against α-amylase and α-glucosidase.
Chemicals and Reagents: α-amylase ex-porcine (SRL148188), α-glucosidase (Maltase) (SRL74854), p-nitrophenyl α-D-glucopyranoside (SRL144969) were purchased from SRL India. Acarbose (A8980) was purchased from Sigma. Starch, NaOH pellets, Na2HPO4, NaH2PO4, NaCl, calcium chloride, dimethyl sulphoxide, Tris base, and other chemicals and solvents were of highest purity grade and purchased from Merck, Mumbai, India. Milli-Q water was used for all the enzymatic assays.
Preparation of Enzyme, Substrate, and Buffer Solutions for α-amylase Assay:
Incubation Buffer: 0.05M Tris Hcl buffer (pH 6.9) containing 0.01M CaCl2.
Enzyme Preparation (20U/ml): A stock solution of 1mg/ml (1mg=20U) α-amylase prepared in Tris-HCl buffer.
Substrate Starch Solution: Starch azure 0.1% in 0.05M Tris-Hcl buffer (pH 6.9) containing 0.01M calcium chloride and boiled for 5-10 min to properly dissolve starch in solution.
Positive Control Acarbose: A stock solution (1mg/ml) prepared in distilled water (Millipore Type I).
Test Sample: A stock solution (1mg/ml) prepared in DMSO and further diluted with water upto 1ml. DMSO concentration was below 2%.
Terminating Solution: 50% acetic acid.
Color Reagent: Iodine solution was prepared by dissolving 0.254g I2 and 4g KI in 1L of distilled water.
Preparation of Enzyme, Substrate, and Buffer Solutions for α-glucosidase Assay:
Incubation Buffer: 0.1M phosphate buffer, pH 6.8
Enzyme Preparation (64U/ml): A working stock of 1mg/ml (1mg=64U) α-glucosidase prepared in phosphate buffer.
Substrate Preparation: 0.5mM of p-nitrophenyl α-D glucopyranoside (PNPG) prepared in phosphate buffer.
Positive Control Acarbose: 1mg/ml dissolved in distilled water (Millipore Type 1).
Test Sample: A stock solution (1mg/ml) prepared in DMSO and further diluted with water upto 1ml. DMSO concentration was below 2%.
Terminating Solution: 0.2M sodium carbonate solution.
α-amylase Inhibition Assay of Crude Leaf Extract: In-vitro α-amylase enzyme inhibition assay was done by following partial modification of the starch-iodine method 15, 16.
A volume of 200µl starch solution (0.1% starch in 0.05M Tris HCl buffer containing 0.01M CaCl2) was taken in a test tube (10 × 100mm) and pre-incubation at 37 °C for 5 min. After pre-incubation, a sample extract solution of 25µl (1mg/ml), 5µl of the α-amylase enzyme (1U) and 270µl of buffer were added to the test tube to make the final reaction mixture of 500µl. The reaction tube was further incubated at 37 °C for 10minutes and stopped by adding 500µl of 50% acetic acid. The reaction mixture was then centrifuged at 3000 rpm for 5 min at 4°C. The upper centrifuged part of the reaction mixture was transferred into a clean and dry test tube. The reaction mixture was allowed to develop color by adding 1ml of iodine solution followed by vortexing for 30 sec. The absorbance was measured at 565nm in a spectrophotometer (Multiskan GO, Thermo-Scientific, Finland). Acarbose was used as standard inhibitor drug for α-amylase. Appropriate sample blanks and controls were included for each sample treatment. All reactions were performed in triplicate. The inhibition (%) of α-amylase activity was calculated by using the formula.
α -amylase inhibition (%) = Sample OD × 100/ Control OD
[Sample OD= Sample OD- Sample blank OD; Control OD = OD of the control reaction without inhibitor-blank OD].
Dose-Response Analysis of α-amylase Inhibition Assay: The significant Clerodendrum species (showed >50% inhibition) were further selected for dose-dependent analysis by taking different con-centrations i.e., 10-100µl≈20-200µg/ml, respectively.
α-glucosidase Inhibitory Assay of Crude Leaf Extract: The α-glucosidase enzyme inhibitory assay was performed in a chromogenic method in which enzyme activity was estimated by measuring yellow color developed due to release of p-nitrophenyl after cleavage of glycosidic linkage of substrate p-nitrophenyl α-D glucopyranoside (PNPG)4. 25μl of sample solution was premixed with 25μl of the enzyme (0.5U) and incubated at 37 °C ± 1 °C for 10 min. After incubation, 25μl of the substrate (0.5mM, p-nitrophenyl α-D glucopyranoside) was added to the reaction mixture and incubated at 37 °C ± 1 °C for 30 min. The reaction was terminated by adding 100μl of 0.2M sodium carbonate solution. The amount of p-nitrophenol released from PNPG was quantified on a 96 well microplate at 405nm in a microplate reader (Multiskan GO, Thermo-Scientific, Finland). Appropriate sample blanks and controls were included for each sample treatment. Acarbose was used as a standard inhibitor drug for α-glucosidase. All reactions were performed in triplicate. The inhibition (%) of α-glucosidase activity was calculated by using the formula.
α-glucosidase inhibition (%) = Control OD-Sample OD × 100/ Control OD
[Control OD = OD of the control reaction without inhibitor-blank OD; Sample OD = Sample OD- Sample blank OD].
Dose-Response Analysis of α-glucosidase Inhibition Assay: The significant Clerodendrum species (showed >50% inhibition) were further selected for dose-dependent analysis by taking different concentrations i.e. 5µl to 30µl≈67µg/ml to 400µg/ml respectively.
IC50 Calculation: The half-maximal inhibitory concentration (IC50) is a measure of the effectiveness of a substance in inhibiting a specific biological or biochemical function. IC50 values were calculated by using an online tool (www.ic50.tk) from the dose-response curve for both α-amylase and α-glucosidase assay.
RESULTS:
Diversity and Ethnobotany of Clerodendrum Species in NER: Present investigation on the taxonomic diversity of Clerodendrum species revealed the rich diversity with the occurrence of 12 numbers of species (C. bracteatum, C. colebrookianum, C. inerme, C. indicum, C. japonicum, C. philippinum, C. serratum, C. speciosum, C. splendens, C. thomsonii, C. viscosum and C. wallichii) from NER Table 1, Fig. 1.
Literature survey covered a total of 60 numbers of reports particularly on ethnomedicine of Clerodendrum species resulted in ten species representing 37 diseases used by 26 tribal communities in entire NER while field survey analysis recorded 9 Clerodendrum species representing 16 diseases used by 13 tribal communities of NER. In contrast, the single species, C.thomsoniae was found to be used as ornamental Table 2.
TABLE 1: DETAILS OF TWELVE CLERODENDRUM SPECIES, THEIR VERNACULAR NAMES, AND PLACE OF COLLECTION FROM NORTHEASTERN REGION
S. no. | Clerodendrum species | Vernacular name (Tribe/NE state) | Place of the collection (NE States) |
1 | C.bracteatum Wall. ex Walp. | Dom-bhetai (Assamese, Bodo Kachari/AS); Atsuksuba (Naga/NL) | Lower Subansiri, Papum Pare (AR) |
2 | C. colebrookianum Walp.
|
Tapen, Poto, Dringi (Adi, Nishi, Nocte, Singpho, Khampti, Tangsa /AR); Nefaphu (Assamese, Bodo Kachari/AS); Jaren, Sia-long, Dien-ja-rem-kyntheri, Jhr-khtung, Yay-iong / Khasi, Jaintia, Garo/ML); Kutab-manbi, Kuthab (Manipuri/MN); Phuinum (Mizo/MZ); Orematong, Umrem (Naga/NL) | Lower Subansiri, Papum Pare (AR); Lakhimpur, Kamrup (AS); Aizwal (MZ); East Imphal, Senapati (MN); Dimapur (NL); Ri Bhoi (ML) |
3 | C. indicum (L.) Kuntze | Akal bih (Assamese/AS); Charoidong (Manipuri/MN);Bamus gach (Garo/ML) | Lakhimpur (AS) |
4 | C. inerme (L.) Gaertn. | - | Papumpare (AR); Lakhimpur (AS) |
5 | C. japonicum (Thunb.) Sweet | Horaiphul (Assamese/AS) | Papumpare (AR); Lakhimpur (AS) |
6 | C. philippinum Schauer | -
|
Changlang, Lohit, Papumpare (AR); Lakhimpur (AS); East Imphal (MN) |
7 | C. serratum (L.) Moon | Nangal bhanga, Teuri-longphlang (Assamese, Kachari/AS); Bharung (Apatani/AR); Sam-seng, Hursymet, Rilong-phlang (Garo, Khasi/ML) | Lower Subansiri, Papumpare (AR); Lakhimpur (AS); Ri Bhoi (ML) |
8 | C. thomsoniae Balf.f. | - | Papumpare (AR) |
9 | C. viscosum Vent. | Purimoli (Nishi/AR); Bhet tita, Dhopa tita, Reiwang, Makhna, Lwkhna, Mwkhwna (Assamese, Kuki, Bodo/AS); Chuikuima (Reang/TR); Dieng-Jarem-Synrang, Sam-makhi (Khasi, Garo/ML); Tokolam Naga/NL) | Changlang, Lohit, Papumpare, (AR); Lakhimpur (AS); Dimapur (NL) |
10 | C.wallichii Merr. | - | East Khasi Hill (ML) |
11. | C. speciosum Dombrain | - | Lakhimpur (AS); Papumpare (AR) |
12. | C. splendens G.Don | - | Lakhimpur (AS); Papumpare (AR) |
AR-Arunachal Pradesh; AS-Assam; MN-Manipur; MZ-Mizoram; ML-Meghalaya; NL-Nagaland
FIG. 1. CLERODENDRUM SPECIES IN NATURAL HABITAT (1) C. BRACTEATUM (2) C. COLEBROOKIANUM (3) C. INERME (4) C. INDICUM (5) C. JAPONICUM (6) C. PHILIPPINUM (7) C. SERRATUM (8) C. SPECIOSUM (9) C. SPLENDENS (10) C. THOMSONII (11) C. VISCOSUM (12) C. WALLICHII
TABLE 2: CLERODENDRUM SPECIES USED IN DIFFERENT AILMENTS AND DISEASES BY VARIOUS TRIBES OF NORTHEASTERN REGION (LITERATURE SURVEY REPORT)
Species | Diseases/
ailments |
NE State | Tribe/
Communities |
Parts used | Mode of uses |
C. bracteatum | Brain tonic | AS | Bodo | R | Juice 26 |
Diarrhea and dysentery | ML | Mizo | L,R | Decoction 27 | |
Fever | AS | Bodo | R | Juice 26 | |
C. colebrookianum | Fever | NL | Naga | mixed with dried and pounded meat of tortoise and decoction 28 | |
Headache | AR | Hill Miri | L | brushing around the forehead 29 | |
Blood pressure | AR | Tangsa | L, TW | boiled in water and mixed with a few ground pieces of Allium sativum L. and salt, are prescribed orally as soup or decoction for a consecutive period of three months, twice a day, on alternate days at 200g per dose 30 | |
AR | Bangni | L | decoction or juice given orally 31 | ||
AR | Nishi | L | Decoction 32 | ||
AR | Apatani | L | boiled or infusion is taken orally 33 | ||
AR | Hill Miri | L | Juice 29 | ||
AR | Memba | L | Decoction 34 | ||
AR | Adi | L | decoction taken 3-4 teaspoonful twice daily 2 | ||
AR | Adi | L | 35 | ||
AR | Monpa | L | decoction with sugar 36 | ||
AS | Hmar | L | leaf juice 37 | ||
AS | Jaintia, Lusai | L | taken raw 38, 39 | ||
AS | Dimasa Kachari | L | leaf extract 40 | ||
AS | Moran | L | Decoction 41 | ||
AS | Zeme | L | Soup 42 | ||
AS | Mishing | L | Boil 43 | ||
AS | Assamese | TW | Boil 44 | ||
AS | Barman | L | Boil 45 | ||
AS | Barman | L | Boil 46 | ||
NL | Naga | L, TW | eaten raw or in soup; boiled in water and the extract is taken daily for few days 28, 47 | ||
ML | Mizo | L | Decoction 48, 38 | ||
ML | Khasi | L | Decoction 49 | ||
ML | Khasi, Jaintia | L | Extract 50 | ||
NL | Naga | L | Decoction of dry leaf 51 | ||
NL | Naga, Kuki | L | Decoction 52 | ||
Malarial fever | NL | Naga | R, B, L | decoction, eaten raw or in soup 28, 53 | |
Reduce weight | AR | Hill Miri | L | - 29 | |
Rheumatism | ML | Khasi, Garo | L | - 53 | |
AS, ML, NL | Khasi, Jaintia, Naga | L | made into paste and massaged for a long time in rheumatic pain and gout 54 | ||
Stomachache | AR | Adi, Hill Miri | L | - 28, 29 | |
Abdominal pain | AS | Tai-Ahom | L | three teaspoonful leaf extract is mixed with small amount of common salt and is taken thrice daily 55 | |
Anthelmintic | AS | Assamese | L | - 53 | |
Antidote | NL | Naga | L | infusion of leaves mixed with bark paste of the “menpan plant” is drunk 28 | |
Blood purifier | AS | Lushai | L | - 39 | |
Colics in infants | ML | Mizo | L | juice of 5 ml twice daily 48 | |
Cough | AR | Adi, Nishi | R | - 2 | |
Diabetes | AS | Jaintia | L | taken raw 38 | |
AS | Barman community | L | Decoction 45 | ||
AS | Zeme | L | Decoction 42 | ||
ML | Mizo | L | Decoction 48 | ||
Diarrhoea and dysentery | AR | Adi, Nishi | L | Juice 53 | |
AS | Dimasa Kachari | R | Extract 40 | ||
Gastric disorders | AR | Bangni | L, S | decoction or juice 31 | |
Fever | AR | Adi | L | boiled and extract 53 | |
Heart trouble | NL | Naga | L, TW | eaten raw or in soup 28 | |
C. hastatum | Skin infection | AS | Assamese | L | paste of 3-4 leaves applied on infected skin area for 8-10 hours 56 |
C. indicum | Vermifuge | AR | Adi | L | - 2 |
Cough | AR | Adi, Nishi | L | - 53 | |
AS | Mikir | L | smoke of dry leaf 13 | ||
Fever | AR | Adi | L | - 2 | |
Rheumatism | AS | - | L | - 57 | |
Asthma | AR | Adi, Nishi | R | - 2 | |
AS | Assamese | R | two teaspoonfuls juice twice daily regularly for a month 58 | ||
Diabetes | MN | Meitei | L | boiled extract along with Justicia adhatoda leaves 59 | |
Jaundice | AR | - | R | soaked in water for overnight and extract taken orally for 7-15 days 60 | |
C. paniculatum | Typhoid | TR | Reang | R | cut into pieces and pounded together with the roots of Tamarindus indica and Ananas comosus and made into paste. The decoction of the mixture is taken internally twice a day 61 |
Fever | TR | Reang | L | juice taken orally 62 | |
C. philippinum | Body pain | SK | - | R | Juice 63 |
Headache | SK | - | R | Juice 63 | |
C. serratum | Cephalalgia and ophthalmania | AR | Adi, Miri | L | - 2 |
Dropsy | AR | Adi, Miri | S | - 2 | |
Fever | AR | Adi, Miri | L | - 2 | |
AS | Jaintia | WP, L | ground with water 38 | ||
Headache | AR | Hill Miri | L | brushing around the forehead 29 | |
Rheumatism | AR | Nishi, Adi | R | - 2 | |
Malaria | AS | - | R | Extract 64 | |
Irregular menstruation | NL | Naga | L | Decoction 28 | |
Jaundice | AR | L | Juice 60 | ||
C. villosum | Liver disorder | NL | Naga | WP | decoction of the plant 28 |
To kill lice | NL | Naga | WP | juice applied on the scalp 28 | |
C. viscosum | Blood purifier | AR | Singpho | L | Boil 33 |
AR | Nishi | F, L | - 32 | ||
Skin diseases and tumor | AS | Assamese | R | - 65 | |
MZ | Mizo | L,R | juice applied externally 48 | ||
Snake bite | AS | Assamese | SH | - 66 | |
Toothache | AS | Bodo | SH | Chewed 67 | |
Vomiting | AS | Sarania | L | crushed to make juice and one teaspoonful taken internally 68 | |
Asthma | AS | Jaintia | L | raw 38 | |
Abdominal pain | AS | - | L | - 65 | |
Body inflation | AS | Bodo | L | Juice twice daily for 3-4 days 69 | |
Cut and wounds | AS | Hmar | L | Paste 37 | |
Malarial fever | AS | Nath | L | Infusion 70 | |
AS | Assamese | SH | 66 | ||
AS | Assamese | L | Decoction 64 | ||
NL, ML | Naga, Khasi | WP | decoction of whole plant along with black pepper 54 | ||
Deworming | AS | Barman | L | - 45 | |
AS | Bengali | L | mixed with rice flour 46 | ||
Diabetes | AS | Jaintia | L | Raw 38 | |
AS | Bengali | L | Juice 46 | ||
MN | Meitei | L | Boil 59 | ||
Diarrhoea and dysentery | AS | Khasi | L | Juice 71 | |
AS | Bodo | 72 | |||
AS | Jaintia, Lushai | R | crushed and decoction taken orally thrice a day 67 | ||
AS | Jaintia, Lushai | L | Boil 46 | ||
MZ | Mizo | L | Juice 64 | ||
Scabies | MZ | Mizo | R | Decoction 48 | |
Dog bite, snakebite | TR | Reang | L | paste 62 | |
C. wallichii | Skin infection | ML | Khasi, Jaintia | L | pounded with slaked lime and applied on skin infections 73 |
Abdominal tumor | MZ | Mizo | R
|
paste of roots mixing with leaves of Ardisia paniculata, Claoxylon khasianum, Phlogacanthus thyrsiflorus applied externally every day for 7 days 74 |
B- Bark; F- Flower; L- Leaf; R- Root; S- Seed; S-Stem; T-twig; SH-Shoot; WP- Whole plant; TW-Twig. AS-Assam; AR-Arunachal Pradesh; MN-Manipur; MZ-Mizoram; ML-Meghalaya; NL-Nagaland; TR-Tripura; SK-Sikkim.
Among the species, C. colebrookianum has been found to be used in a total of 17 different diseases where a maximum of 21 northeast tribes used for curing of blood pressure, 7 tribes used for diabetes, 4 tribes used for rheumatism, 3 tribes used for diarrhea and dysentery, fever respectively reported from a literature survey. While in field survey, the species has been used in 13 different diseases where a maximum number of informants (90) belonging to 12 different tribal communities used for controlling of blood pressure, 4 tribes used in gastric trouble, fever, dysentery and abdominal pain, 3 tribes in stomach trouble, diabetes, heart trouble, headache and cough, 2 tribes in malaria, 1 tribe in jaundice and sinusitis respectively Table 3. Young and tender leaves were used in the majority of diseases and ailments in both literature and field survey Fig. 2. Mode of administration was found to be leaf boil in both literature and field survey Fig. 3.
FIG. 2: DIFFERENT PLANT PARTS OF CLERODENDRUM SPECIES USED IN TRADITIONAL MEDICINE (LITERATURE SURVEY AND FIELD SURVEY)
FIG. 3: DIFFERENT WAYS OF HERBAL PREPARATION OF CLERODENDRUM SPECIES USED IN TRADITIONAL MEDICINE (LITERATURE SURVEY AND FIELD SURVEY)
TABLE 3: ETHNO MEDICINAL USES OF CLERODENDRUM SPECIES BY VARIOUS TRIBAL COMMUNITIES OF NORTHEASTERN REGION (FIELD SURVEY REPORT)
Species | Parts used | Diseases/
ailments |
Mode of preparations | NE State | Tribes/Communities |
C. bracteatum | L | fever | juice | AR | Nishi |
C. colebrookianum | L | blood pressure | boil with bamboo shoot, garlic; dry leaves chewed | AR, AS, MN, NL, MZ, ML | Nishi, Adi, Apatani, Assamese, Khasi, Mishing. Mizo, Bodo, Khamti, Naga, Sonowal Kachari, Tagin |
L, F | gastric trouble | boil with bamboo shoot | AR, ML | Nishi, Adi, Apatani, Khasi | |
L | diabetes | boil | AR | Nishi, Adi, Apatani | |
L, F | stomach trouble | boil | AR, ML | Nishi, Adi, Apatani, | |
L, FR | fever | boil | AR, NL, MZ | Nishi, Adi, Apatani, Mizo | |
L | malaria | boil | AR | Nishi, Adi | |
L | jaundice | boil | ML | Khasi | |
L | heart trouble | boil | AR | Nishi, Adi, Apatani, | |
L | diarrhoea and dysentery | boil | AR | Nishi, Adi, Apatani, Tagin | |
L | cough | boil | AR | Nishi, Adi, Apatani, | |
L | abdominal pain | boil | AR, MZ | Nishi, Adi, Apatani, Mizo | |
L | headache | boil | AR | Nishi, Adi, Apatani, | |
L | sinusitis | juice | ML | Khasi | |
C. inerme | L | cuts and wounds | paste | AR | Nishi, Adi, Apatani |
C. indicum | L | jaundice | juice | AR | Nishi, Adi |
R | asthma | juice | AR, AS | Adi, Nishi, Assamese | |
L | fever | boil | AS | Assamese, Bodo | |
C. japonicum | L | typhoid | juice | AS | Bodo |
C.philippinum | L | cuts and wounds | paste | AR | Nishi, Adi, Apatani |
L | cough | juice | AS | Bodo, Khasi | |
C. serratum | L | jaundice | decoction | AS | Mishing |
L | cuts and wounds | paste | AS | Deori | |
R | cuts and wounds | paste | AR, AS | Nishi, Khasi | |
L | fever | juice | AR | Nishi | |
C. thomsoniae | - | ornamental | - | - | - |
C. wallichii | L | abdominal pain | juice | ML | Khasi |
F- Flower; FR-Fruit; L- Leaf; R- Root, AS-Assam; AR-Arunachal Pradesh; MN-Manipur; MZ-Mizoram; ML-Meghalaya; NL-Nagaland
Screening of α-amylase Inhibition Properties: The crude ME of four species (C. serratum, C. japonicum, C. viscosum and C. indicum) have shown significant inhibition of enzyme activity (>50%) while the other 3 species (C. philippinum, C. colebrookianum, and C. inerme) have shown insignificant inhibition activity (<50%) as compared to the standard inhibitor of α-amylase, acarbose (ACB) (80%) Fig. 4.
FIG. 4: α-AMYLASE INHIBITION PROPERTIES OF SEVEN CLERODENDRUM SPECIES AND STANDARD DRUG ACARBOSE. T-BARS ON THE HISTOGRAM REPRESENT STANDARD DEVIATION (SD)
Dose-Response Analysis of α-amylase Inhibition Assay: The inhibition was found to be increased with increasing concentrations in all species with the lowest inhibition by C. serratum (28% in 10µl) to highest inhibition by C. indicum and C. serratum (97% in 100µl) respectively. The inhibition activity for standard drug acarbose (ACB) was between 7 to 85% in 10 to 100µl concentrations Fig. 5.
FIG. 5: COMPARISON OF α-AMYLASE INHIBITION PROPERTIES OF FOUR CLERODENDRUM SPECIES AND STANDARD DRUG ACARBOSE (ACB) AT DIFFERENT CONCENTRATIONS (10-100µL≌20-200µG/ML).T-BARS ON THE HISTOGRAM REPRESENT STANDARD DEVIATION (SD)
Screening of α-glucosidase Inhibition Properties: The crude ME of 3 species were found to possess significant inhibition (>50%) of α-glucosidase activity. C. viscosum was found to show the highest level of inhibition (73%) followed by C. inerme (68%) and C. colebrookianum (59%) in comparison with standard drug, acarbose (86%). Four species (C. japonicum, C. serratum, C. philippinum and C. indicum) showed no significant inhibition of the enzyme activity Fig. 6.
FIG. 6: α-GLUCOSIDASE INHIBITION PROPERTIES OF SEVEN CLERODENDRUM SPECIES AND STANDARD DRUG ACARBOSE. T-BARS ON THE HISTOGRAM REPRESENT STANDARD DEVIATION (SD)
Dose-Response Analysis of α-glucosidase Inhibition Assay: The α-glucosidase inhibition percentage was found to be increased with increasing concentrations of the leaf extract. C. viscosum showed the highest inhibition in all concentrations (23% in 5µl to 83% in 30µl). Similarly, C. colebrookianum and C. inerme also displayed enzyme inhibition properties between the lowest of 18% (5µl) to a maximum of 80% (30µl), respectively Fig. 7.
FIG. 7: COMPARISON OF α-GLUCOSIDASE INHIBITION PROPERTIES OF 3 CLERODENDRUM SPECIES AND STANDARD DRUG ACARBOSE AT DIFFERENT CON-CENTRATIONS (5-30µL ≈ 67-400µg/mL). T-BARS ON THE HISTOGRAM REPRESENT STANDARD DEVIATION (SD)
50% Inhibition Concentration (IC50) of Leaf Extracts: The IC50 values showed ranged from a minimum of 45µg/ml of C. serratum to a maximum of 71µg/µl of C. japonicum as compared to positive control acarbose (79µg/ml) for α-amylase. On the other hand, IC50 values for α-glucosidase ranged from 47µg/ml of C. viscosum to a maximum of 184µg/ml of C. inerme as comparison to acarbose (244µg/ml). Among the species, crude ME of C. serratum and C. viscosum showed the lowest IC50 values of α-amylase (45µg/ml) and α-glucosidase (47µg/ml) Table 4.
TABLE 4: IC50 VALUES OF LEAF EXTRACTS WITH POTENTIAL Α-AMYLASE AND Α-GLUCOSIDASE INHIBITION PROPERTIES FROM CLERODENDRUM SPECIES. VALUES WITHIN PARENTHESIS SHOW SD OF MEAN
Clerodendrum
species |
IC50 (µg/ml) | |
α-amylase | α-glucosidase | |
C. serratum | 45 (±-4.8) | - |
C. indicum | 47(± 8.1) | - |
C. japonicum | 71 (± 8.0) | |
C. viscosum | 59 (± 1.2) | 47 (±1.3) |
C. colebrookianum | - | 175 (±0.86) |
C. inerme | - | 184 (±1.2) |
Acarbose | 79 (±1.3) | 244 (±0.78) |
DISCUSSION: From the present observation, it has been found that many Clerodendrum species growing in NER are continuously used by tribal communities for the treatment of many diseases in the form of special preparations or as vegetables. C. colebrookianum, C. inerme, C. indicum, C. serratum were found to have multi medicinal properties. To validate the traditional claims associated with Clerodendrum species, many scientific investigations were carried out by using in-vitro and in vivo assays. In the present study, 4 species of Clerodendrum (C. serratum, C. japonicum, C. viscosum, C. indicum) exhibited significant α-amylase inhibition properties while 3 other species (C. viscosum, C. colebrookianum, C. inerme) displayed significant α-glucosidase inhibition properties.
Previous studies have reported crude ME of C. viscosum possessed hypoglycemic property against streptozotocin and alloxan-induced diabetes in wister rats 17, 18. C. serratum and C. inerme leaf extract were reported to have significant blood glucose-lowering potential in STZ-induced diabetic rats 19, 20, 21. These findings are in agreement with significant in-vitro α-amylase, and α-glucosidase inhibition properties of the methanol extracts of the three species (C. serratum, C. viscosum and C. inerme) reported in the present study.
The present study of C. colebrookianum methanol leaf extract was found to show moderately high percentage inhibition of α-glucosidase activity. A study on experimentally induced insulin resistance rats also revealed significant ameliorating role of aqueous leaf extract of C. colebrookianum 22. C. colebrookianum, C. indicum, C. inerme, C. japonicum, C. philippinum, C. viscosum and C. serratum are reported to contain various bioactive compounds such as acacetin, acteoside, apigenin-6-C-β-l-fucopyranoside, apigenin-7-0-glucoside, isoacteoside, isoquercetin, hispidulin, hispidulin 7-O-glucuronide, kaempferol, luteolin, oleanic acid, quercetin, rutin, etc. 23, 24, 25, 1.
All these compounds isolated from other medicinal plants reported having antidiabetic properties; however isolation, particularly from Clerodendrum species together with antidiabetic properties, has not been established.
CONCLUSION: Therefore, it is concluded that four Clerodendrum species were found to possess highly significant antidiabetic properties as revealed by in-vitro α-amylase, and three other species have shown significant α-glucosidase inhibition properties. Despite large information available on phytochemical compounds and their correlation with antidiabetic properties from different species of the genus Clerodendrum, there is scarce information on the phytochemical characterization of the above species with potential antidiabetic properties reported in this study. A further investigation into phytochemical profiling and evaluation of their role in hypoglycemic activities of selected species, specially C. viscosum and C. serratum under in-vitro and in-vivo conditions may help in the identification of lead molecule for therapeutic use in treatment and management of diabetes.
The hypoglycemic effect of Clerodendrum species may be due to the presence of more than one antidiabetic bioactive compound or their synergistic properties. Further bioassay-guided fractionation and in-silico screening bioactive compounds from Clerodendrum species may serve as target inhibitors in the treatment and management of diabetes mellitus in the near future.
ACKNOWLEDGEMENT: The authors gratefully acknowledge the financial support and infrastructure facility of DBT, Govt. of India in the form of NER-Twinning project (HC-118, 2011) & Institute level Biotech HUB (IBTHub), Dept. of Forestry, NERIST (Deemed University), Nirjuli, Arunachal Pradesh. Also grateful to Botanical Survey of India, Arunachal Pradesh for identification of Clerodendrum species and thank local guides, villagers of NER for cooperation, and sharing the valuable information on medicinal uses together with support during the fieldwork.
CONFLICTS OF INTEREST: Nil
REFERENCES:
- Shrivastava N and Patel T: Clerodendrum and Healthcare: An overview. Medicinal Aromatic Plant Science and Biotechnology 2007; 1(1): 142-50.
- Srivastava RC and Choudhary RK: Species diversity and Economic importance of the family Verbenaceae in Arunachal Pradesh. Bulletin of Arunachal Pradesh Research 2008; 24(1&2): 1-21.
- West IC: Radicals and oxidative stress in diabetes. Diabetic Medicine 2008; 17(3): 171-80.
- Laishram S, Sheikh Y, Moirangthem DS, Deb L, Pal BC, Talukdar NC and Borah JC: Antidiabetic molecules from Cycas pectinata traditionally used by the Maiba Maibi. Phytomedicine 2015; 22(1): 23-26.
- Butler MS: The role of natural product chemistry in drug discovery. Journal of Natural Products 2004; 67(12): 2141-53.
- Adefegha SA and Oboh G: Antioxidant and inhibitory properties of Clerodendrum volubile leaf extracts on key enzymes relevant to non-insulin-dependent diabetes mellitus and hypertension. Journal of Taibah University for Science 2016. doi.org/10.1016/j.jtusci.2015.10.008.
- Liu Q, Hu HJ, Li PF, Yang YB, Wu LH, Chou GX and Wang ZT: Diterpenoids and phenylethanoid glycosides from the roots of Clerodendrum bungei and their inhibitory effects against angiotensin-converting enzyme and α-glucosidase. Phytochemistry 2014; 103: 196-202.
- Sneha JA and Chaudhary S: Alpha-amylase inhibitory and hypoglycemic activity of Clerodendrone multiflorum stems. Asian Journal of Pharmaceutical and Clinical Research 2011; 4(2): 99-102.
- Kang DG, Lee YS, Kim HJ, Lee YM and Lee HS: Angiotensin-converting enzyme inhibitory phenyl-propanoid glycosides from Clerodendron trichotomum. Journal of Ethnopharmacology 2003; 89(1): 151-54.
- Liu Q, Hu HJ, Li PF, Yang YB, Wu LH, Chou GX and Wang ZT: Diterpenoids and phenylethanoid glycosides from the roots of Clerodendrum bungei and their inhibitory effects against angiotensin-converting enzyme and α-glucosidase. Phytochemistry 2014; 103: 196-202.
- Hooker JD: Flora of British India. L. Reeve and C. Ltd. London, Edition 4, 1885: 589-96.
- Kanjilal UN, Kanjilal PC, De RN and Das A: Flora of Assam. Government of Assam, Shillong, Edition 3, 1939: 485-93.
- Deori C, Roy D, Talukdar SR, Pagag K and Sarma N: Diversity of the genus Clerodendrum linnaeus (Lamiaceae) in Northeast India with special references to Barnadi Wildlife Sanctuary, Assam. Pleione 2013; 7(2): 473-88.
- Martin GJ: Ethnobotany, a methods manual. Springer US, Edition 1, 1995.
- Hamdan II, Afifi F and Taho MO: In-vitro alpha amylase inhibitory effect of some clinically used drugs. Pharmazie 2004; 59(10): 799-801.
- Hansawasdi C, Kawabata J and Kasai T: Alpha-amylase inhibitors from Roselle (Hibiscus sabdariffa) tea. Bioscience Biotechnology Biochemistry 2000; 64(5): 1041-43.
- Ahmed F and Rahman MS: In-vitro antidiabetic activity of Clerodendrum viscosum. Journal of Pharmacreations 2014; 1(2): 47-51.
- Das S, Bhattacharya S, Prasanna A, Sureshkumar RB, Pramanik G, Haldar PK: Preclinical evaluation of antihyperglycemic activity of Clerodendron infortunatum leaf against streptozotocin-induced diabetic rats. Diabetes Therapy 2011; 2(2): 92-100.
- Kar MH, Trupti RS and Sagar KM: Antidiabetic activity of Clerodendrum serratum (L.) moon leaves in streptozotocin-induced diabetic rats. Asian Journal of Pharmaceutical and Clinical Research 2014; 7(6): 260-63.
- Pandey R, Yadav SK and Gupta SK: Anti-diabetic activity of Clerodendrum (or Clerodendron) inerme using in vivo and in-vitro studies, novel science. International Journal of Pharmaceutical Science 2012; 1(6): 298-302.
- Panigrahi BK, Mishra SK and Sahu SK: Antidiabetic effects of Clerodendrum inerme (L) Gaertn. Asian Journal of Pharmacy and Pharmaceutical Research 2015; 4(2): 248-56.
- Jadeja RN, Thounaojam MC, Devkar RV and Ramachandran AV: Clerodendrum glandulosum extract prevents in-vitro human 106 LDL oxidation and oxidized LDL induced apoptosis in human monocyte derived macrophages. Food and Chemical Toxicology 2011; 49: 1195-1202.
- Gao LM, Wei XM and He YQ: Studies on chemical constituents in leaf of Clerodendron fragrans. Zhongguo Zhong Yao Za Zhi 2003; 28(10): 948-51.
- Gunasegaran, R, Recio MC, Alcaraz MJ and Nair AGR: Additional constituents from Clerodendrum indicum, (Verbenaceae). Pharmazie 1993; 48(2): 151-52.
- Sankara SS and Ramachandran NAG: Scutellarin and hispidulin-7-0-glucuronide from the leaves of Clerodendrum indicum and Clerodendrum infortunatum. Phytochemistry 1973; 12(5): 1195.
- Basumatary S, Ahmed K and Deka M: Some medicinal plant leaves used by Boro (tribal) people of Goalpara district, Assam. Natural Product Radiance 2004; 3(2): 89.
- Rai PK and Lalramnghinglova H: Lesser known ethnomedicinal plants of Mizoram, North East India: An Indo-Burma hotspot region. Journal of Medicinal Plants Research 2010; 4(13): 1301-07.
- Changkija S: Folk medicinal plants of the Nagas in India. Asian Folkore Studies, Nanzan University, 1999; 58(1): 205-30.
- Tag H and Das AK: Ethnobotanical notes on Hill Miri tribe of Arunachal Pradesh. Indian Journal of Traditional Knowledge 2004; 3(1): 80-85.
- Nath SC and Bordoloi DN: Diversity of Economic Flora in Arunachal Pradesh: Plant folk medicines among the Chakma, Singpho and Tangsa tribals. GB Pant Institute of Himalayan Environment & Development, Almora, Vol I, 1993: 179-89.
- Gupta V: Traditional medicinal plants of the Bangnis of East Kameng district, Arunachal Pradesh. Journal of Economic and Taxonomic Botany 2006; 30: 310-19.
- Bhuyan LR: Some plants used as medicines by the Nishi tribe of Arunachal Pradesh: A preliminary study. Journal of Economic and Taxonomic Botany 2003; 27(2): 447-50.
- Khongsai M, Saikia M, Saikia SP and Kayang H: Ethnomedicinal plants used by different tribes of Arunachal Pradesh. Indian Journal of Traditional Knowledge 2011; 10(3): 541-46.
- Tag H and Das AK: Ethnobotanical notes on Hill Miri tribe of Arunachal Pradesh. Indian Journal of Traditional Knowledge 2004; 3(1):80-85.
- Rethy P, Singh B, Kagyung R and Gajurel PR: Ethnobotanical studies of Dehang-Debang biosphere reserve of Arunachal Pradesh with reference to Memba tribe. Indian Journal of Traditional Knowledge 2010; 9(1): 61-67.
- Namsa ND, Mandal M, Tangjang S and Mandal SC: Ethnobotany of the Monpa ethnic group at Arunachal Pradesh, India. Journal of Ethnobiology and Ethnomedicine 2011; 7: 31.
- Nath M and Choudhury MD: Ethno-medico-botanical aspects of Hmar tribe of Cachar district, Assam (Part-I). Indian Journal of Traditional Knowledge 2010; 9(4): 760-64.
- Sajem AL and Gosai K: Traditional use of medicinal plants by the Jaintia tribes in North Cachar Hills district of Assam, Northeast India. Journal of Ethnobiology and Ethnomedicine 2006; 2(33): 126.
- Sajem AL and Gosai K: Ethnobotanical investigations among the Lushai tribes of North Cachar Hills district of Assam, Northeast India. Indian Journal of Traditional Knowledge 2010; 9(1): 108-13.
- Tamuli P and Sharma P: Ethno-medico-botany of the Dimasa Kachari of North Cachar Hills district of Assam. Indian Journal of Traditional Knowledge 2010; 9(4): 718-20.
- Buragohain J: Ethnomedicinal plants used by the ethnic communities of Tinsukia district of Assam, India. Recent Research in Science and Technology 2011; 3(9): 31-42.
- Tamuli P and Saikia R: Ethno-medico-botany of the Zeme tribe of North Cachar Hills district of Assam. Indian Journal of Traditional Knowledge 2004; 3(4): 430-36.
- Gogoi M, Upadhyaya S, Borkataky M and Kardong K: Use of indigenous plants in traditional health care systems by Mishing tribe of Upper Assam. SIBCOLTEJO 2010; 5: 92-101.
- Gogoi R and Das MK: Observations on some weeds of medicinal importance in the Brahmaputra valley of Assam. Journal of Economic and Taxonomic Botany 2003; 27(2): 434-41.
- Das AK and Sharma GD: Ethnomedicinal uses of plants by Manipuri and Barman communities of Cachar district, Assam. Journal of Economic and Taxonomic Botany 2003; 27(2): 421-29.
- Das AK, Dutta BK and Sharma GD: Medicinal plants used by different tribes of Cachar district, Assam. Indian Journal of Traditional Knowledge 2008; 7(3): 446-54.
- Jamir TT, Sharma HK and Dolui AK: Folklore medicinal plants of Nagaland, India. Fitoterapia 1999; 70: 395-401.
- Sharma HK, Chhangte L and Dolui AK: Traditional medicinal plants in Mizoram, India. Fitoterapia 2001: 72(2): 146-61.
- Hynniewta SR and Kumar Y: Herbal remedies among the Khasi traditional healers and village folks in Meghalaya. Indian Journal Traditional Knowledge 2008; 7(4): 581-86.
- Chhetri RB: Trends in ethnodomestication of some wild plants in Meghalaya, Northeast India. Indian Journal of Traditional Knowledge 2006; 5(3): 342-47.
- Deb L, Singh Kh. R, Singh BK and Thongam B: Some ethno-medicinal plants used by the native practitioners of Chandel district, Manipur, India. International Research Journal of Pharmacy 2011; 2(12): 199-200.
- Pfoze NL, Kumar Y and Myrboh B: Survey and assessment of ethnomedicinal plants used in Senapati district of Manipur State, Northeast India. Phyto-pharmacology 2012; 2(2): 285-311.
- Jain SK and Saklani A: Cross-cultural ethnobotanical studies in northeast India. Ethnobotany 1992; 4: 25-38.
- Singh SP, Tripathi S and Shukla RS: Ethnomedicinal heritage for bioprospecting and drug development in North Eastern states of India. Journal of Economic and Taxonomic Botany 2003; 27(2): 384-95.
- Kalita D and Phukan B: Some ethnomedicines used by the Tai Ahom of Dibrugarh district, Assam, India. Indian Journal of Natural Product Research 2010; 1(4): 507-11.
- Baishya AK and Bora PJ: Cross community ethno-medico botany of Dibru-Saikhowa Biosphere Reserve, Assam. Bulletin of Botanical Survey of India 2007; 49(1-4): 128.
- Kalita PC and Barthakur SK: Studies on some highly utilized important medicinal plants among rural people of Madankamdev hill region, Assam. Journal of Economic and Taxonomic Botany 2010; 34(2): 257-61.
- Nath KK, Deka P, Borkakoti S and Borthakur SK: Traditional remedies for respiratory disorders from Assam, India. Pleione 2008; 2(2): 211-16.
- Khan MH and Yadava PS: Antidiabetic plants used in Thoubal district of Manipur, Northeast India. Indian Journal of Traditional Knowledge 2010; 9(3): 510-14.
- Shankar R, Rawat MS, Deb S and Sharma BK: Jaundice and its traditional cure in Arunachal Pradesh. Journal of Pharmaceutical and Scientific Innov 2012; 1(3): 93-97.
- Shil S and Choudhury MD: Indigenous knowledge on healthcare practices by the Reang tribe of Dhalai district of Tripura, North East India. Eth leaflets 2009; 13(6): 775-90.
- Shil S and Sharma GD: A few medicinal plants from Reang tribals of North Tripura. Journal of Economic and Taxonomic Botany 2003; 27(2): 451-56.
- Krishnan B and Singh S: Ethnobotanical observation in Sikkim. Journal of Economic Taxonomic Botany 1987; 9:1-7.
- Begum SS and Hynniewta TM: Medicinal plant resources of Nameri Nation Park, Assam, India. Annals of Forestry 2007; 15(1): 62-70.
- Nath PC, Sarmah, R, Arunachalam A and Barbhuiya AR: Non timber forest products (NTFPs): Potential and their conservation in Dihing-Patkai Wildlife sanctuary (Jaypore Reserve Forest) of Assam. Journal of Non Timber Forest Products 2006; 13: 17-23.
- Gogoi R and Das MK: Observations on some weeds of medicinal importance in the Brahmaputra valley of Assam. Journal of Economic Taxonomic Botany 2003; 27(2): 434-41.
- Bora PJ: A study on ethnomedicinal uses of plants among the Bodo tribe of Sonitpur district, Assam, India. Journal of Economic Taxonomic Botany 1999; 23: 604-08.
- Sharma SK, Devi B and Bhattacharjya DK: Ethnomedicinal uses of plants by the Sarania tribe Nalbari district of Assam. Journal of Economic Taxonomic Botany 2006; 30: 133-39.
- Saikia B, Borthakur SK and Saikia N: Medico-ethnobotany of Bodo tribals in Gohpur of Sonitpur district, Assam. Indian Journal of Traditional Knowledge 2010; 9(1): 52-54.
- Sikdar M and Dutta U: Traditional phytotherapy among the Nath People of Assam. Ethnomed 2008; 2(1): 39-45.
- Baruah MK, Choudhury PD and Sharma GC: Ethno-medicinal plants used by the Khasi Tribe of Cachar district, Assam. Journal of Economic and Taxonomic Botany 2006; 30: 110-14.
- Das AK, Dutta BK and Sharma GD: Study of medicinal plants used by the different tribes/communities of Cachar district of Assam-I. Journal of Economic and Taxonomic Botany 2006; 30: 294-309.
- Kharkongor P and Joseph J: Folklore medico-botany of rural Khasi and Jaintia tribes in Meghalaya. Oxford & IBH Publishing Co., New Delhi, 1981: 124-36.
- Lalramnghinglova H: Ethno-medicinal plants of Mizoram. Bishen Singh Mahendra Pal Singh, Dehra Dun 2003, First Edition, 111-14.
How to cite this article:
Kalita J, Singh SS and Khan ML: Ethnomedicinal values and antidiabetic potential of Clerodendrum spp. occurring in Northeastern region. Int J Pharm Sci & Res 2020; 11(10): 5112-24. doi: 10.13040/IJPSR.0975-8232.11(10).5112-24.
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Article Information
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English
IJPSR
J. Kalita *, S. S. Singh and M. L. Khan
Department of Biotechnology, Gauhati University, Guwahati, Assam, India.
janmoni.kalita@gmail.com
18 October 2019
05 April 2020
25 August 2020
10.13040/IJPSR.0975-8232.11(10).5112-24
01 October 2020