EVALUATION OF ANTI-INFLAMMATORY AND OTHER BIOLOGICAL ACTIVITIES OF FLAVONOID BASED CREAM FORMULATION FOR TOPICAL APPLICATION USING IN VITRO MODEL

Biologically active compounds identified as flavonoids, glycosides, alkaloids, saponins, terpenoids and polyphenolics from plant extracts possess health promoting properties. The use of natural antioxidants in the treatment of various skin diseases has gained utmost importance in recent years. Therefore, present study was designed and conducted with the aim to evaluate the anti-inflammatory activity of cream formulation containing fruit extracts of Musa acuminata, Psidium guajava and Pyrus communis using in vitro model. Fruit of M. acuminata, P. guajava and P. communis were extracted by cold percolation, comprising of ethanol, methanol and distilled water. The cream formulation and the reference drug (Diclofenac sodium) of varying concentrations were incubated with fresh egg albumin under specified experimental conditions and subjected to determination of absorbance and viscosity to assess the in vitro anti-inflammatory activity. Linear regression analysis was used to calculate IC₅₀ value. The results revealed that cream formulation and diclofenac sodium inhibited protein denaturation in a dose-dependent manner. The IC₅₀ value of cream formulation 1254.13±16.91µg/ml and diclofenac sodium was recorded as 845.82±16.94µg/ml at correlation coefficient value (r) of 0.900 and 0.983 respectively. The study gives an idea that the fruits extract can be used as a lead compound for designing a potent topical anti-inflammatory drug.