EVALUATION OF ANTIMICROBIAL ACTIVITY OF DIFFERENT EXTRACTS OF DATURA STRAMONIUM AGAINST MYCOBACTERIUM KANSASII

The aim of this study is to assess the antibacterial efficacy of the Datura stramonium seeds and leaves extracts against Mycobacterium kansasii. The antimicrobial experiments were performed using five doses (0.25µg/ml, 0.50µg/ml, 1µg/ml, 2µg/ml, 4µg/ml) of seeds and leaves extracts. The results showed notable inhibitory effects on Mycobacterium kansasii. The results indicated that the extract of D. stramonium exhibited action against all the tested bacterial isolates, with the level of activity varying depending upon the concentration. The hexane extract of seeds DSS-1H exhibited antibacterial activity, with mean zones of inhibition measuring 98.94±50.25.%, 120.98±56.92%, 120.499±69.60%, 125.752±133.18% and 89.494±180.63%, at the doses of 0.25, 0.50, 1, 2, and 4 µg/ml and while treating with DSS-2A the percent cell viability of the microbial cell was found to be 128.02±25.64%, 86.79±14.95%, 34.61±16.33%, 26.29±29.76% and 32.56±19.48% at the doses of 0.25, 0.50, 1, 2 and 4µg/ml, respectively, with incubation period of 48 hours. Similarly, the microbial cells were treated (0.25, 0.50,1,2,4 µg/ml) with DSS-3ET with incubation period of 48 hours, indicating a more substantial decrease in cell proliferation (75.09±39.45, 60.16±44.46, 28.19±20.73, 42.24±32.80% and 46.93±69.00%). In a similar manner the percent cell viability of plant extract (DSL-M and DSL-E) also found to be decreased upon treatment with the same concentration (0.25, 0.50, 1, 2, 4µg/ml) with incubation period of 48 hours, which were 146.05±15.24%, 117.63±36.36%, 59.88±95.54, 16.45±58.76 and 42.89±7.23% and 89.52±23.73%, 102.93±28.55%, 60.66±18.13, 58.86±28.90% and 50.91±8.83% respectively, as compared to the control. The findings of this study provided evidence for the conventional utilization of wildly growing plant Datura stramonium and emphasize the necessity for further comprehensive research to explore potential alternatives to current antibacterial medications.