EVALUATION OF ANTIOXIDANT ACTIVITY AND QUANTIFICATION OF α–ASARONE IN RHIZOMES OF ACORUS CALAMUS L. USING HPTLC FINGERPRINTING
AbstractThis study aimed to analyze antioxidant activity and quantify alpha-asarone by using High-performance thin-layer chromatography (HPTLC). Alpha-asarone is a secondary metabolite in hydroalcoholic (1:1) rhizome extracts of Acorus calamus L. (AC), a medicinal plant being used around the globe traditionally. Sweet flag, AC is a member of the Acoraceae family, and is commonly used unaided as well as in conjunction with other plants in Chinese traditional medicine and the Indian system of medicine. It has sparked a lot of curiosity and has proven to be useful in managing many diseases. Acorus calamus and/or its bioactive phytochemical α-asarone, is a well-known medication in a traditional system with various properties. Antioxidants are extremely essential compounds that can defend the body from harm caused by oxidative stress generated by free radicals. HPTLC is a viable option for expanding chromatographic fingerprints in order to identify medicinal plant’s key active components. At 100µg/mL, the antioxidant activity of AC demonstrated 52.59 percent and increasing to 56 percent at 500µg/mL. HPTLC fingerprinting profiles revealed the presence of α-asarone is one of phytoconstituent present in hydroalcoholic extract of rhizome extracts of AC. The retardation factor (RF) was observed at 0.38, and the quantification is done at a wavelength of 260 nm. The amount of α-asarone was found to be 263.6 µg in 1.0 mg of plant extract.
Article Information
46
482-496
6473 KB
430
English
IJPSR
Sunishtha Kalra, Rohit Malik, Saurabh Bhatia, Aditya Bhushan Pant and Govind Singh *
Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak, Haryana, India.
drgovind.pharma@mdurohtak.ac.in
18 May 2022
30 August 2022
18 November 2022
10.13040/IJPSR.0975-8232.14(1).482-96
01 January 2023