EVALUATION OF HAEMATINIC ACTIVITY OF POLYHERBAL FORMULATION IN HgCl2 INDUCED ANAEMIA IN LABORATORY ANIMALSAbstract
Anaemia constitutes serious health problem in many countries. It is widespread public health problem, and the fourth leading cause of hospital admissions and the second factor contributing to death. Anaemia is characterized by low count of haemoglobin. The empirical use of herbal preparations in the treatment of anaemia dates from ancient times. Despite the obvious effectiveness and efficacy of iron supplementation, there are certain limitations which include gastrointestinal side effects like nausea, constipation, vomiting, and stained teeth. HgCl2 was administered for induction of anaemia in rats by oral route at 9mg/kg for 30 days simulaneousaly with test & standard drug. After 1 week blood was withdrawn to check all haematological parameters. The animals were treated with standard and test drug. After 2, 3, 4 weeks blood was withdrawn to check haematological & biochaemical parameters. Preliminary phytochemical screening by various tests was done. Acute oral toxicity was also done at 2000mg/kg and 5000mg/kg.HgCl2 induced significant decrease in the blood parameters indicating anaemia which was significantly increase in test and standard treated animals. Acute toxicity studies in mice established the oral LD50 greater than 5000 mg/kg. Haema tablet assessed for the haematinic activity by using: HgCl2 induced anaemia in rats. In the present study, the pharmacological evaluation of Haema tablet has done for the haematinic effect. In the present study, it can be concluded that animals treated with standard & Haema tablet in dose 500 mg/kg of body weight was effective.
Pradnya N. Jagtap*, Vaishali R. Undale and A.V. Bhosale
PDEA’s Seth Govind Raghunath Sable College of Pharmacy, Saswad, Tal- Purandar, Dist- Pune, Maharashtra, India
14 April, 2013
10 June, 2013
14 August, 2013
01 October, 2013