EVALUATION OF IN-VITRO ANTIOXIDANT AND NEPHROPROTECTIVE POTENTIALS OF CRINUM ASIATICUM BULBS AGAINST GENTAMICIN INDUCED NEPHROTOXICITY IN ALBINO RATS

Objective: To study the in-vitro antioxidant as well as nephroprotective potentials of Crinum asiaticum bulbs’ethanolic extract in contrast to gentamicin-induced nephrotoxicity in albino rats. Materials and Methods: The ethanolic extract of Crinum asiaticum bulbs subjected to preliminary phytochemical analysis to determine the phytoconstituents and evaluation of antioxidant activity was done using in-vitro methods like DPPH as well as lipid peroxidation assays using ascorbic acid as standard. In-vivo nephroprotective activity was estimated by utilising gentamicin induced nephrotoxicity in albino rats. Results: It was revealed by the preliminary phytochemical analysis that phenolics, tannins, steroids, terpenoids, and flavonoids components are presented. The promising results obtained by in-vitro antioxidant activity promoted us to evaluate gentamicin induced nephrotoxicity in rats. Gentamicin impeded nephrotoxicity in rats as proved by extremely substantial (p<0.001) rise of blood urea, serum creatinine and uricacid in Group II in comparison to Group I. Also, administration of Gentamicin in group II caused oxidative stress in rats proposed by considerable increase in LPO level as well as considerable decrease in GSH level. While administration of EECA in group III to V respectively caused a dose dependent significant decrease (p<0.01) of uric acid, serum creatinine, as well as blood urea in comparison to group II. There is a significant increase in in-vivo antioxidant status (p<0.01) in catalase and GSH levels, however there is a substantial reduction in LPO levels (p<0.01) in group III to V. Conclusion: The promising results suggested that Crinum asiaticum bulbs’ ethanolic extract has nephroprotective activities in contrast to gentamicin induced nephrotoxicity in rats.