EVALUATION OF IN-VITRO ANTIPLASMODIAL ACTIVITY OF SELECTED ETHNOBOTANICALLY IMPORTANT MEDICINAL PLANT EXTRACTSAbstract
Purpose: A serious cause of morbidity and mortality, malaria remains a major public health concern. Failure of available treatments due to the rapid emergence of drug-resistant parasite strains emphasizes the need for a continuous search for new, safe, accessible, and affordable drug treatments. Methods: In this context, an ethnobotanical survey was conducted, different parts of seventeen medicinal plants were collected, and their methanolic extracts were prepared. These extracts were evaluated for anti-plasmodial activity against two strains of Plasmodium falciparum: chloroquine-sensitive 3D7 and resistant INDO using SYBR-Green I assay. The extracts were also assessed for cytotoxic effects against HEK293 mammalian cell lines using MTT assay. Results: Two plant extracts: Albizia lebbeck and Tecomella undulata exhibited good activity with IC50 ranging from 10-20µg/ml. Among others, eight showed moderate activity (IC50= 20.1- 50µg/ml), while eight extracts either showed poor activity or were not active upto IC50=100µg/ml against Pf3 D7. The extracts with good to moderate effects showed equipotent effects against Pf INDO and were non-toxic to mammalian cell lines with selectivity indices ranging from 2.3 to >13.3. This study validated the traditional usage of the selected plants especially leaves of A. lebbeck and T. undulata. Conclusion: The results obtained have presented a starting material for identification and purification of active compounds that might provide an alternative drug therapy to fight against malaria.
Cheryl Sachdeva, Sunny Dhir, Amit Kaushik, Vineet Kaswan, Yashika Walia and Naveen Kumar Kaushik *
Amity Institute of Virology and Immunology, Amity University Uttar Pradesh, Sector-125, Noida, Uttar Pradesh, India.
23 June 2022
02 September 2022
19 November 2022
01 February 2023