EVALUATION OF POLYHERBAL FORMULATION FOR ANTIPYRETIC ACTIVITY ON SMALL ANIMAL MODEL

Background: Fever is a part of a complex biological response due to pathogens, bacterial infection, damaged cells, drugs, toxins, etc. Fever is currently treated by NSAIDs. Unfortunately, NSAIDs damage the liver and kidney, inhibit COX-1, which affects the digestive system, and increase the risk of a blood clot. Therefore, the developments of potent antipyretic drugs from medicinal herbal plants are now under consideration. Polyherbal formulations (PHFs) was become resurgent due to the demand and consumption of herbal drugs. The present research aims to evaluate the antipyretic screening activity of PHFs in albino Wistar rats. Material methods: Different PHFs combinations were formulated using hydro-alcoholic extracts and explored the antipyretic activity of PHFs in albino Wistar rats. Male albino Wistar rats weighing 100-150g were selected and grouped among normal control, standard (Paracetamol; 150mg/kg) and treatment groups (PHF; 100 mg/kg, PHF; 300 mg/kg and PHF; 600 mg/kg) respectively. To check the safety parameters of PHFs extract, Hematological and biochemical tests were also performed. Results: PHFs (100mg/kg, 300mg/kg and 600mg/kg) shows antipyretic effect against brewer’s yeast induced pyrexia in rats. It is noted that the % inhibition is 57.59 % in standard PCM, 43.39%in 100mg/kg, 42.68% in 300mg/kg and 42.66% in 600mg/kg dose. Discussion and Conclusions: The extract of PHFs has shown good antipyretic activity in Wistar rats. The present investigation comprised of pharmacological screening of PHFs extract in lab animals. The chemical constituents of PHFs extract were identified by different qualitative chemical test, and PHFs was selected for the pharmacological screening of antipyretic activity. The PHFs (100mg/kg, 300mg/kg, and 600mg/kg) show a significantly decreased pyretic effect against brewer’s yeast-induced pyrexia in rats compared to the normal group. This suggests its efficacy in preventing hyperthermia. The extract of PHF_S shows important compounds such as carbohydrates, steroids, alkaloids, flavonoids, tannins, and triterpenes which may be responsible for antipyretic activity.