EVIDENCE FOR COMPLEX FORMATION OF IBUPROFEN WITH GO-GHRITA

Present investigation provides evidence for complex formation of Ibuprofen (Ib) with Go-Ghrita (GG).  Ibuprofen binary mixture in (1:0.5), (1:1), (1:2), (1:3) w/w proportions were prepared by adding to molten Go-Ghrita kept over a water bath at 65-70^oC with continuous stirring. Organoleptic and Physico-chemical properties of GG (procured from Magan Sangrahalaya, Wardha) were as per the specifications given in Ayurvedic Pharmacopoeia (AP) and Indian Pharmacopoeia (IP). All proportions were subjected for FT-IR and in-vitro release behaviour (SGF, P^H 1.2 for 2 Hrs and SIF, P^H 6.8 for 7 Hrs) and on basis of it, optimized (1:1) w/w proportions were further analyzed and characterized for Differential Scanning Calorimetry, X-ray diffraction, Nuclear Magnetic Resonance and Scanning Electron Microscopy. Carbonyl stretching of isopropionic acid group to slight higher frequency 1.39 % cm^-1 (increase) and (–C=O) linked (-OH) band stretching to slight lower frequency 1.09 cm^-1 (decrease) shown in FT-IR spectra, supported by in-vitro release of Ibuprofen 99.16±0.17% of (1:1) w/w in sustenance form than remaining. Exclusion of polymorphic modification in X-RD, Slight lowering of 5⁰C Tm and enthalpy change of 5.70 % (loss) in DSC, deviation in chemical shift (ranging from -0.041 to +0.030) as well as selective broadening of signals in NMR and entrapment of Ibuprofen crystals in Ib-GG binary mixture shown in SEM photographs, reflecting possible evidence for the formation of inclusion type complex between Ibuprofen and GG