EVULSIONS SPECTROPHOTOMETRIC DETERMINATION OF CYCLOSERINE IN PHARMACEUTICAL FORMULATIONS WITH BPB AND BCG DYES
AbstractTwo simple and sensitive evulsions spectrophotometric methods have been described for the assay of Cycloserine either in pure form or in pharmaceutical formulations. The developed methods involve formation of coloured chloroform extractable ion – pair complexes of the drug with bromophenol blue (BPB) and bromo cresol green (BCG) in acidic medium. For two methods, the extracted complexes showed absorbance maxima at 408 and 419.5nm. Beer’s law is obeyed in the concentration ranges 2.0-20 and 2.5-25μg/mL with BPB and BCG respectively. The effects of concentration of dye, pH and interference of recipients have been studied and optimized. The limit of detection and quantification has been determined for two methods. These two are very accurate methods of physical and instrumental mode of experiments. Two methods have been validated as per the guidelines of ICH. The methods have been applied to the determination of drug in commercial tablets and results of analysis were validated statistically through recovery studies.
Article Information
40
3005-3011
9315 KB
232
English
IJPSR
M. Ravi *, E. Ramya and K. Padmavati
Department of Science and Humanities, CMR Institute of Technology, Hyderabad, Telangana, India.
ravichemistry1@gmail.com
13 October 2022
05 December 2022
30 April 2023
10.13040/IJPSR.0975-8232.14(6).3005-11
01 June 2023
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