EXPERIMENTAL MODELS TO STUDY TUMOUR ANGIOGENESIS – IN-VITRO, EX-VIVO AND IN-VIVO APPROACH

Angiogenesis is the formation of new blood vessels from preexisting vasculature. It is a key process in some physiological conditions such as wound healing, growth and reproduction. Any disturbance in mechanisms of angiogenesis plays a key role in the pathogenesis of some diseases through the over-proliferation of blood vessels such as cancers, psoriasis, arthritis, retinopathies, obesity, asthma, and atherosclerosis and cardiovascular diseases, which have the highest rates of morbidity and mortality worldwide. Anti-angiogenic therapy has been proved to be a potential cure in the past decades for a number of cancers. Still, antiangiogenic therapy has to be realized to its fullest potential to turn cancer into a dormant disease. Many angiogenic inhibitors have been developed till date, and the main problem is the multiple angiogenic molecules produced by tumors and tumors at different stages of development. Therefore, blocking a single angiogenic molecule has less effect or no effect. Selection of a suitable model for angiogenesis research remains a challenge. Thus, in recent years, several models for evaluation of angiogenesis have been introduced for determining the target molecule. In this article, we briefly reviewed in-vitro, ex-vivo and in-vivo models to evaluate tumor angiogenesis and determine specific targets.