EXTRACTION AND SCREENING OF BIOACTIVE METABOLITES FROM VIGNA MUNGO AGAINST VARIOUS PATHOGENS
AbstractIn the present study, Vigna mungo (blackgram) have been used to investigate bioactive compounds through soxhlet extraction. The obtained methanol extract was evaluated for antimicrobial activity by Kirby-Bauer method against bacterial strains (Enterococci sp., Pseudomonas aeruginosa, Klebsiella sp., Bacillus sp., Staphylococcus aueres)and fungal strains (Aspergillus niger, Candida albicans) on Muller Hinton agar medium and potato dextrose agar medium respectively. The blackgram was analyzed for the presence of proteins through SDS-PAGE and compared with the BSA (66KDa) and Lysine (18KDa) protein markers. The sensitivity of the obtained methanol extract against pathogenic microorganisms was studied with the combined effect of commercially available antibiotics which resulted in good and enhanced antimicrobial effect. The methanol extract of blackgram was also analyzed for bioactive compounds through gas-chromatography mass spectrometry (GC-MS). The GC-MS spectrum revealed bioactive compound with molecular weight of 297.3268 (Heptadecanoic acid, 9-methyl, methyl ester). The active constituents of Vigna mungo was identified through mass spectroscopy analysis act as potential compound for effective antimicrobial and pharmaceutical studies
Article Information
18
428-431
313KB
1356
English
IJPSR
D. Kingsley, G. Ravikumar, R. Chauhan and J. Abraham*
Microbial Biotechnology Laboratory, School of Bioscience and Technology, VIT University, Vellore – 632014, Tamil Nadu, India
jayanthi.abraham@gmail.com
11 September, 2013
21 October, 2013
09 January, 2014
http://dx.doi.org/10.13040/IJPSR.0975-8232.5(2).428-31
01 February, 2014
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