FIRST REPORT ON AMYLASE PRODUCTION BY BACILLUS PUMILUS DS5 ISOLATED FROM AGRICULTURAL FIELD SOILS
AbstractFor the present study, 10 bacterial strains were isolated from agriculture field soils collected from Guntur, Andhra Pradesh, India, and thereafter screened for amylase activity. Among them, the strain DS5 showed a maximum zone of clearance on the starch hydrolysis test was selected for this study. Identification of bacterial strain DS5 was confirmed by 16S rRNA sequencing analysis. The phylogenetic tree constructed on the basis of 16S rRNA sequences revealed that it clustered with the closest members of Bacillus pumilus DS5 and the sequences were deposited in NCBI Gen bank along with accession number MK430437. Further the effect of incubation period, temperature, pH, carbon and nitrogen sources was optimized. Highest amylase production was found after 48 h of incubation at pH 7.0 and 40 ⁰C temperature. Starch proved to be the best carbon source and peptone as nitrogen source for the highest production of amylase by Bacillus pumilus DS5. Supplementation with different nitrogen and carbon source favoured the increased enzyme yield by submerged fermentation.
Article Information
49
2541-2546
656 KB
298
English
IJPSR
D. Silpa * and G. Kranthi Kumar
Department of Microbiology, Maris Stella College, Vijayawada, Andhra Pradesh, India.
dr.dsilpa@marisstella.ac.in
07 September 2022
05 April 2023
18 April 2023
10.13040/IJPSR.0975-8232.14(5).2541-46
01 May 2023
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