FORMULATION AND EVALUATION OF BSA LOADED PLGA MICROPARTICLES
AbstractProtein drug delivery has emerged to be an important area of research in the field of novel drug delivery technology. The objective of the study was to prepare poly (D, L-lactide-co-glycolide) (PLGA) microspheres containing bovine serum albumin (BSA) as a model drug and to evaluate the various physicochemical characteristics of the formulations, namely morphology, particle size, FTIR, DSC, BSA encapsulation efficiency and in-vitro BSA release profile. BSA-loaded microspheres were prepared by double emulsion solvent evaporation method with different BSA: PLGA ratios and at different speeds of homogenization keeping the amount of BSA constant in all the formulations. Out of those 1:10 was selected as a optimized (drug: polymer) for BSA loaded PLGA microspheres, there after internal parameters like volume of inner aqueous phase(2ml), volume of DCM(10ml), concentration of polymer (9.09%),speed of homogenization, were selected as a optimized formulation parameters. Accelerated stability testing was performed with the optimized formulations for a period of eight weeks. The mean particle size and encapsulation efficiency of the microspheres were found to decrease as the speed of homogenization increased. And the same were found to increase simultaneously with increase in the amount of polymer. The in vitro release study showed a slow and steady release pattern of BSA. Accelerated stability studies indicated that formulations here stable during the period of study. Thus, a sustained release formulation of protein loaded PLGA microspheres was developed.
Article Information
15
1013-1021
1065KB
1464
English
IJPSR
Santosh Voruganti*, J. Surya Chandra Padman
Department of Pharmaceutics, Mother Teresa College of Pharmacy, Ghatkesar, Hyderabad, Andhra Pradesh, India
voruganti102@gmail.com
09 November, 2012
12 January, 2013
15 February, 2013
http://dx.doi.org/10.13040/IJPSR.0975-8232.4(3).1013-21
01 March, 2013