FORMULATION AND EVALUATION OF HERBAL TREATMENT OF VITILIGO

Vitiligois depigmentation disorder, and it causes loss of melanocytes. Denaturation of melanocytes caused by oxidative stress, genetics, auto immune response and then synthesis of inflammatory mediators. Vitiligo is classified into two types segmental and non-segmental. There is various treatment to cure vitiligo and some treatment causes side effects. So, through herbal plants we can cure the diseases the herbal plants are Ginkgo biloba, Nigella sativa, Psoralea corylifolia there are plants which has antioxidant properties, anti-inflammatory, these antioxidant helps to neutralizes free radicals and reduce oxidative damage in the skin. Psoralea corylifolia it has ability to stimulate melanin production in the skin through this repigmentaion occurs in the skin. In the pursuit of developing effective treatments for vitiligo, understanding the chemical composition and molecular structure of a topical formulation is essential. GC-MS analysis is used to identify the compound present in polyherbal lotion, FT-IR spectroscopy is used to analzye the functional group present in the lotion, HPLC quantitative analysis is used to determine exact amount of active ingredients present in the polyherbal lotion. The lotion formulation has enriched activity against occident stress and inflammation. Skin irritation test has done for the formulation check whether formulation produce’s edema and redness in the skin. The topical formulation of lotion was prepared and evaluated, and the lotion was prepared for 25 grams. The two in-vitro studies were done H₂O₂ and albumin denaturation assay through this oxidant stress and inflammation will be reduced.