FORMULATION, EVALUATION AND CYTOTOXIC POTENTIAL OF METRONIDAZOLE LOADED POLOXAMER 407 HYDROGEL IN SCC-29 CELL LINESAbstract
The present research work was planned to formulate poloxamer 407 based hydrogel formulations of metronidazole and the evaluation of various parameters like swelling behavior, drug PH stability, in-vitro and in-vivo drug release, and in-vitro cytotoxic activity. Two different concentrations of metronidazole hydrogel formulations were prepared using poloxamer 407 and were assessed by a validated HPLC method for drug content, pH stability, and in-vivo drug release. Further, in-vitro anticancer activity was evaluated using sulphorhodamine B (SRB) assay in SCC29 cell lines. Both the formulations F1 and f2 showed better pH stability at pH 3.5, 5.5 and 6.8. The formulation F1 was able to absorb about 152% of its weight of water within 80 min, whereas F2 absorbed 167.4% of its weight of water and remains constant over 100 min. In-vitro and in-vivo drug release pattern showed half-life at 6 h, AUC0−t 692 and 684ng h/ml, Cmax 1059 and 1142 ng/ml for F1 and F2 respectively. Hydrogel formulation F1 showed improved percentage control growth when compared to F2 hydrogel formulation and metronidazole alone.
P. Katakam, S. R. Banapuram * and S. K. Adiki
Vikas College of Pharmacy, Janagaon, Warangal, Telangana, India.
01 July 2018
08 September 2018
15 September 2018
01 March 2019