GENOMIC STRUCTURAL IDENTIFICATION AND IMMUNOINFORMATICS STUDIES FOR SARS‐COV‐2Abstract
Coronaviruses are a group of viruses that belongs to the Coronaviridae family. These viruses have helical symmetry, enveloped, positive-sense, single-stranded RNA genome with nucleocapsid that causes diseases in birds and mammals. SARS-CoV 2 complete genome has genome size of 29,903 bp, having nine genes identified as Orf1a Polyprotein, Orf1ab Polyprotein, ORF3a Protein, ORF7a Protein, ORF8 Protein, Envelope proteins, Spike Surface glycoprotein, Membrane glycoprotein, and Nucleocapsid Phosphoprotein. Based on the ProtParam result, ORF1a has more molecular weight (489989 daltons), and Envelope protein has a less molecular weight (8365 daltons). The pI is lower in Orf1a polyprotein, Orf1ab Polyprotein, ORF3a Protein, ORF8 Protein, and Spike Surface glycoprotein) and high in ORF7a Protein, Envelope proteins, Membrane glycoprotein, and Nucleocapsid Phosphoprotein. The Orf1a Poly-protein, Orf1ab Polyprotein, ORF3a Protein, Envelope proteins, Spike Surface, and Membrane glycoprotein are stable. The higher aliphatic index and more hydrophobicity of membrane glycoprotein suggest an increase in the thermostability of protein might favor an increase in its solubility. The vaccines like EIAV vaccine gp45, anti-SARS m396 Antibody, EIAV vaccine gp45, and E protein of the Japanese encephalitis live attenuated vaccine virus are less effective than Curcumin for SARS-CoV 2. Hence better vaccines are to be developed for respiratory viruses like SARS-CoV 2 in the future.
D. S. V. G. K. Kaladhar
Department of Microbiology and Bioinformatics, UTD, Atal Bihari Vajpayee University, Bilaspur, Chhattisgarh, India.
01 April 2020
20 January 2021
30 January 2021
01 April 2021