GREEN SYNTHESIS, CHARACTERIZATION AND ANTI-MICROBIAL ACTIVITY OF SILVER NANOPARTICLES PRODUCED USING IPOMOEA AQUATICA FORSK LEAF EXTRACT

Objective: The aim of the present study is to investigate the synthesis and characterization of Silver nanoparticles (AgNPs), and their antimicrobial effect on gram positive and gram negative bacteria by using hydro alcoholic leaf extract of Ipomoea Aquatica Forsk (HAEIA).

Material and Methods: AgNPs were prepared by green synthesis process from 1mM AgNO₃ solution the aqueous silver ions when exposed to the HAEIA were reduced and stabilized over long periods of time resulting in the green synthesis of surface functionalized silver nanoparticles. The bio-reduced silver nanoparticles were appropriately characterized by using UV-Vis spectroscopy, Particle size distribution and Zeta potential. Antibacterial activity of synthesized silver nanoparticles (AgNPs) was carried out on both Gram negative and Gram positive bacteria and compared with standard antibiotic Ciprofloxacin.

Result: The particle size of bio synthesized silver nanoparticles is found to be in the range of 77 – 624 nm with polydispersity index (PDI) of 0.207 clearly indicates that the particles are in very narrow size distribution. The zeta potential value of -6.16 mV that the nanoparticles prepared is stable.

Conclusion: The PDI value clearly indicates that the particles are in very narrow in size distribution. The zeta potential value indicates that the nanoparticles prepared are stable in the extract. Hence from this study it was concluded that green synthesized silver nanoparticles are more stable and also have wide spectrum of anti-microbial activity.