GREEN SYNTHESIS OF ZINC OXIDE NANOPARTICLES USING PRUNUS DOMESTICA (PLUM) FRUIT EXTRACT ITS CHARACTERIZATION, ANTIMICROBIAL AND ANTIOXIDANT STUDIES

Production of nanoparticles using plants is a new area of interest. Plum fruit extract was used for the biosynthesis of zinc oxide nanoparticles. Green synthesis of zinc oxide nanoparticles are considered as a eco-friendly methodusing materials from plants and other natural sources without using anyharmful chemicals. Plum fruit has many medicinal values such as antioxidant activity, cardiovascular activity, anti-inflammatory and antimicrobial effect. Present paper discuss about the green synthesis of zinc oxide nanoparticles using Prunus domestica (plum) fruit extract, Its characterization by analytical studies, antimicrobial and antioxidant studies. Plum fruit extract also subjected to phytochemical screening. The active constituents present in Plum were identified as alkaloids, glycosides, phenolics, flavonoids, proteins and carbohydrates. Nano particles were characterized by UV-Visible Spectroscopy, FT-IR and SEM studies. In the UV visible spectrometer absorption peak was observed at 362 nm, which is specific for zinc oxide nanoparticles. Synthesized zinc oxide nanoparticles were subjected to FT-IR analysis to detect the various characteristic functional group associated with the synthesized nanoparticles. The peaks indicate the characteristics functional group present in the zinc oxidenanoparticles. Synthesized zinc oxide nanoparticles were subjected to scanning electron microscopy (SEM) to determine the morphology and particle size of nanoparticles. It can be seen that all the particles were in spherical shape with particle size distribution from 77.5nm to 110 nm. The variation in size may be due to decreased amount of capping agents. The antimicrobial properties of the particles were determined using agar well diffusion and the disc diffusion method using Bacillus subtills and E. coli bacteria. The green synthesised ZnO NP showed antimicrobial activity against both Bacillus subtills and E. coli bacteria. The zinc oxide nanoparticles showed more activity against E. coli bacteria compared to Bacillus subtills. The antioxidant activity of nanoparticles was performed using Hydrogen peroxide scavenging and total phenolic content method, indicates the presence of antioxidant properties.