HELICOBACTER PYLORI INFECTION: A BIOINFORMATIC APPROACH
AbstractHelicobacter pylori causative agent of acid peptic disease is a microaerophilic, spiral-shaped, gram-negative bacteria found in the gastric epithelium that may also lead to complications such as chronic gastritis, mucosa-associated lymphoid tissue (MALT) lymphoma, gastroduodenal ulcer and adenocarcinoma in stomach. Plantbioactives have always been potential therapeutics. Usage of modern bioinformatic tools plays a vital role in exploiting the potentials of alternative therapeutic molecules in managing diseases like peptic ulcers and their complications. The in-silico evaluation was carried out using molecular docking of the quorum sensing proteins of H. pylori with the ligand β-sitosterol obtained from the silver nanoparticles of Acorus calamus L. Among several given quorum sensing proteins the molecular interaction with the ligand β-sitosterol showed a high binding affinity with DnaA, PhnB, ToxB and Sip proteins. The results obtained from molecular interaction study revealed that the ligand β-sitosterol will be readily taken up by the organism, thereby facilitating easy inhibition or inactivation of quorum sensing molecules ToxB, DnaA, PhnB, and Sip, making it a novel therapeutic alternative to treat H. pylori infections.
Article Information
17
5469-5483
2036
829
English
IJPSR
A. Prasad, G. Shruthi, P. Sushma, A. S. Jain, D. Chandan, M. N. N. Prasad, S. P. Kollur, C. Srinivasa and C. Shivamallu *
Division of Biotechnology and Bioinformatics, Department of Water & Health, Faculty of Life Sciences, JSS Academy of Higher Education & Research, Sri Shivarathreeshwara Nagara, Mysore, Karnataka, India.
chandans@jssuni.edu.in
12 February 2020
29 March 2020
31 March 2020
10.13040/IJPSR.0975-8232.11(11).5469-83
01 November 2020
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