HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC DETERMINATION OF PARACETAMOL, DICLOFENAC, AND CAFFEINEIN COMBINED TABLET DOSAGE FORM
AbstractA new simple High-Performance Thin Layer Chromato-graphic (HPTLC) method for determining Paracetamol, Diclofenac and Caffeine in combined tablet dosage form has been developed and validated. The mobile phase selected was Ethyl acetate: Methanol: ammonia (8: 2:0.1 v/v/v) with UV detection at 263 nm. The retention factor for Paracetamol, Diclofenac, and caffeine was found to be 0.639 ± 0.018, 0.267 ± 0.026, and 0.447 ± 0.026, respectively. The method was validated with respect to linearity, accuracy, precision and robustness as per the International Conference on Harmonization (ICH) guidelines. Results were found to be linear in the concentration range of 1000-6000 ng/band for Paracetamol 200-1200 ng/band for Diclofenac and 100-600 ng/band for caffeine, respectively. The method has been successfully applied to analyze drugs in pharmaceutical formulation. The % assay (Mean ± S.D.) was found to be 98.64 ± 0.184 for paracetamol, 97.17± 1.074 for Diclofenac and 98.27± 0.86 caffeine. The method can be used for routine analysis of these drugs in combined tablet dosage forms in quality-control laboratories.
Article Information
31
3952-3956
631 KB
449
English
IJPSR
Vasudha S. Bavadekar * and Sheetal D. Chaudhari
Department of Pharmaceutical Chemistry, Modern College of Pharmacy, Nigdi, Pune, Maharashtra, India.
vasudha.bavadekar@gmail.com
23 November 2022
14 February 2023
27 May 2023
10.13040/IJPSR.0975-8232.14(8).3952-56
01 August 2023
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