HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC) METHOD FOR THE DETERMINATION OF SOME AMINO ACIDS FROM TWO SPECIES OF MARCHANTIA
AbstractThe objective of the present study is to develop a rapid HPTLC method for the identification, separation and quantification of some amino acidsfrom two species of Marchantia. Three different mobile phases were used for the separation and estimation of eight amino acids (alanine, glycine, methionine, threonine, valine, leucine, histidine and isoleucine). TheHPTLC analysis of Marchantia palmata and M. nepalensis, showed the presence of all the eight amino acids. The content of all the amino acids in Marchantia nepalensis were higher than M. palmata. Of all the amino acids, the glycine content (0.417 mg/g dw in Marchantia nepalensis and 0.287 mg/g dw in M. palmata) was found to be maximum and valine content (0.053 mg/g dw in Marchantia nepalensis and 0.031 mg/g dw in M. palmata) was found minimum. The presence and content of amino acids in the liverworts has been for the first time evaluated by a modern technique like HPTLC. This method provides an important tool for the quantitative evaluation of important amino acidsandgives more accurate results than the conventional ones reported in the literature.
Article Information
34
5328-5333
435KB
1565
English
IJPSR
Kanchna Devi, Sunita Kapila* and Anju Rao
Department of Botany, Panjab University, Chandigarh, Pin-160014, India.
s_kapila0802@yahoo.co.in
09 May, 2014
21 July, 2014
15 August, 2014
http://dx.doi.org/10.13040/IJPSR.0975-8232.5(12).5328-33
01 December 2014
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