HIGH PRESSURE LIQUID CHROMATOGRAPHIC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF ACYCLOVIR IN BULK AND MARKETED FORMULATION
AbstractA simple, selective, rapid and economical High Performance Liquid Chromatography (HPLC) method was developed for estimation of Acyclovir in bulk and marketed formulation. Chromatographic separation and estimation was achieved in a run time of 6.482 minutes on Hemsil C18– 5U (250 × 4.6 mm, 5μm) using Acetonitrile and pH adjusted water (6.74) in the ratio of 10:90 v/v at flow rate of 0.5ml/min. UV detector set at 254nm was used for detection. The linearity range for Acyclovir was found to be 4-40 µg/ml with coefficient of linear regression greater than 0.9948. The method was validated as per International Conference on Harmonization (ICHQ2 (R1) 2005) guidelines for accuracy, precision, specificity, LOD & LOQ, linearity and robustness. The method was successfully applied for estimation of Acyclovir from bulk and marketed formulation for routine analysis
Article Information
48
2194-00
442
1526
English
IJPSR
Suchita V. Ghumre *, Varsha M. Jadhav and Vilasrao J. Kadam
Bharati Vidyapeeth’s College of Pharmacy, C.B.D Belapur, Navi Mumbai, Maharashtra, India.
suchitaghumre08@gmail.com
18 December, 2015
27 January, 2016
13 February, 2016
10.13040/IJPSR.0975-8232.7(5).2194-00
01 May 2016
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