HIGH PRESSURE LIQUID CHROMATOGRAPHIC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF ACYCLOVIR IN BULK AND MARKETED FORMULATIONAbstract
A simple, selective, rapid and economical High Performance Liquid Chromatography (HPLC) method was developed for estimation of Acyclovir in bulk and marketed formulation. Chromatographic separation and estimation was achieved in a run time of 6.482 minutes on Hemsil C18– 5U (250 × 4.6 mm, 5μm) using Acetonitrile and pH adjusted water (6.74) in the ratio of 10:90 v/v at flow rate of 0.5ml/min. UV detector set at 254nm was used for detection. The linearity range for Acyclovir was found to be 4-40 µg/ml with coefficient of linear regression greater than 0.9948. The method was validated as per International Conference on Harmonization (ICHQ2 (R1) 2005) guidelines for accuracy, precision, specificity, LOD & LOQ, linearity and robustness. The method was successfully applied for estimation of Acyclovir from bulk and marketed formulation for routine analysis
Suchita V. Ghumre *, Varsha M. Jadhav and Vilasrao J. Kadam
Bharati Vidyapeeth’s College of Pharmacy, C.B.D Belapur, Navi Mumbai, Maharashtra, India.
18 December, 2015
27 January, 2016
13 February, 2016
01 May 2016