HPLC, FTIR AND GC-MS ANALYSIS OF ETHYL ACETATE EXTRACT OF RED SEAWEED HYPNEA FLAGELLIFORMIS GRAVILLE EX J. AGARDH 1851

The experiment was carried out to investigate the phytochemicals constituents of the red seaweed Hypnea flagelliformis (Greville ex J. Agardh. 1851) from Tuticorin coastal waters, Gulf of Mannar, southeast coast of India; by using HPLC, FTIR, and Gas Chromatography-Mass Spectrometry. The selected seaweed was extracted with ethyl acetate (by soxhlet extraction method), furthermore subjected to HPLC, FTIR, and GC-MS analysis to reveal the phytochemical constituents. Totally five peak values were found in HPLC analysis. Among the five, three are a prominent peak at 2.243, 2.447, and 30.787 min, and the area percentages were 23.0, 45.4, and 30.6, respectively. The IR spectrum of ethyl acetate extract shows nine peaks corresponding to the wavenumber 3403.16, 2923.88, 2853.49, 2361.67, 1717.49, 1460.98, 1378.04, 1248.82, and 1042.45 cm^‑1. In the GC-MS analysis, a total of nineteen peaks were obtained, and the chromatograph showed 19 individual compounds. From all the nineteen compounds, three major compounds identified based on area percentage, which includes 1,4-eicosadiene (29.01%), n-tetracosanol-1(14.06%), and tetradecyl trichloro-acetic acid (7.90%). Further, these novel bioactive structures can be optimized by using combinatorial chemistry generating new drug designs for future research.