HPLC METHOD FOR ESTIMATION OF DRUG RELEASE OF ENTACAPONE IN ENTACAPONE, LEVODOPA AND CARBIDOPA TABLETSAbstract
Today’s generic drugs manufacturing industry need robust analytical methods for analysis of generic drug products to provide best quality pharmaceutical formulation. Entacapone, Levodopa and Carbidopa tablets is such a product which is used for the treatment of Parkinson’s disease. This paper presents a robust HPLC method that has been developed for estimation of % drug release of Entacapone in this multi component drug formulation. HPLC column used for separation was Cosmosil 5PE-MS 150mm x 4.6 mm with 5 micron particle size. Combination of phosphate buffer pH 2.5, acetonitrile and methanol was used for mobile phase in isocratic mode with UV detection at 280 nm. The method was validated to ensure suitability of the method for quantitative determination of % drug release of Entacapone in presence of Levodopa and Carbidopa in this triple drug combination pharmaceutical formulation. Proposed method was found to be specific, precise, robust, accurate and linear in range 0.066 to 0.33 mg/mL of Entacapone.
Prasoon Bhatnagar*, Deepak Vyas, Shailendra Kumar Sinha and Archana Gajbhiye
Research Scholar, Department of Chemistry, Sir Padampat Singhania University, Bhatewar, Udaipur, Rajasthan, India.
15 August, 2016
07 December, 2016
24 February, 2017
01 April, 2017