IDENTIFICATION OF GENES EXPRESSED DURING WATER STRESS IN FOUQUIERIA SPLENDENS SSP. BREVIFLORA
AbstractWe identified genes in Fouquieria splendens ssp. breviflora (Fouquieriaceae) that were expressed due to water deficits. RNA was extracted from dehydrated stem samples that were collected directly from Camargo, Querétaro following hydration for two weeks. The plants were grown in two states, drought and hydrated. We then performed subtractive hybridization. The obtained sequences were inserted into a plasmid and sequenced. Analyses were performed using the Blastx and Expasy ScanProsite database. These results revealed the expression of genes that corresponded to the RNA polymerase II β-subunit of chloroplast, rpoC2; the cell binding site known as tripeptide RGD; catalase; and chitinase class I and III. These expressed genes are important because they are involved in the response to oxidative stress and the defense response and development in Fouquieria splendens ssp. Breviflora
Article Information
7
1396-1400
380
1472
English
Ijpsr
R. I. M. Sánchez, M. D. P. Crisóstomo Vázquez, M. A. V. Alberto, J. L. M. Sánchez and E. J. Cardoso
Laboratory of Parasitology Research, Dr. Márquez #162 Col. Doctores, México
jimenezce@yahoo.com.mx
17 August, 2014
25 November, 2014
30 December, 2014
10.13040/IJPSR.0975-8232.6(4).1396-00
01 April, 2015
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