IMMUNOLOGICAL AND BIOCHEMICAL CHARACTERIZATION OF GLUTATHIONE-S-TRANSFERASE IN PARTHENIUM POLLINOSIS AND ITS CROSS-REACTIVITY WITH BIRCH POLLINOSIS FOR CLINICAL RELEVANCE
AbstractPollinosis is one of the most common allergic diseases in Asia, Central and Northern Europe as well as in North America. Several proteins in parthenium pollen have already been characterized as allergens causing sensitization and the development of allergic symptoms in atopic individuals. Glutathione-S-transferase are a phylogenetically widespread enzyme family mainly involved in cellular detoxification processes and have already been described as allergens in house dust mite and German cockroach. However, no data about the role of parthenium-GST in plant pollen allergy were available so far, for this purpose we evaluated its immunological and biochemical features. In biochemical assays b-GST was not enzymatically active, whereas p-GST demonstrated high enzymatic activity. In summary, IgE antibodies level did not show significant raise after incubation neither in the VT20 nor in the Bet v1 group while significant raise is observed with IgG antibodies. No cross-reactivity between p-GST-specific Abs and b-GST was observed. In contrast, GST-specific IgG clearly recognized GST in VT20.
Article Information
47
3533-3538
414
1060
English
IJPSR
Praveen Kumar Vemuri *, Bhavana Talluri and Gayathri Panangipalli
Centre for Genomics and Proteomics, Department of Biotechnology, K L University, Vaddeswaram, Guntur District, Andhra Pradesh, India
vemuripraveen@kluniversity.in
22 March, 2016
05 July, 2016
13 July, 2016
10.13040/IJPSR.0975-8232.7(8).3533-38
01 August 2016
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