IMPACT OF DIFFERENT CARBON SUPPLEMENT ON EXTRACTION OF C-PHYCOCYANIN (C-PC) FOLLOWED BY POLY-β-HYDROXYBUTYRATE (PHB) FROM NOSTOC MUSCORUMAbstract
The extraction of poly-β-hydroxybutyrate (PHB) and C-phycocyanin (C-PC) from Nostoc muscorum revealed that PHB yield after C-PC extraction was not found differ significantly as without C-PC extraction. Under photoautotrophic growth condition, the test cyanobacterium accumulates PHB and C-PC with a maximum yield of 40 and 57 mg l-1 respectively at the stationary phase, i.e. on day 21 of incubation. The most significant enhancement in C-PC yield up to 146 mg l-1 was recorded in 0.4 % fructose supplementation. High C-PC and PHB yield was observed in 0.4% fructose supplementation followed by 0.4% glucose supplementation. Here, in this study we show, that PHB yield after C-PC extraction was not affected significantly and C-PC yield was found enhanced under 0.4 % carbon source (fructose and glucose) supplementation. Significant Biomass and PHB yield depicted under supplementation of glucose, fructose, maltose and sucrose was due to boost in growth. Thus, present study demonstrates the extraction of both the products, i.e. PHB and C-PC is possible.
Vrijesh Kumar Yadav * and Vikash Kumar Yadav
Indian Institute of Technology, Kharagpur, 721302, West Bengal, India.
23 November, 2016
13 January, 2017
14 January, 2017
01 June, 2017