IMPACT OF PROBIOTICS LACTOBACILLUS RHAMNOSUS AND LACTOBACILLUS CASEI ON LIPOGLYCEMIC AND ANTIOXIDATIVE STATUS OF DIABETIC RATS

Probiotics are live microorganisms, which when administered in adequate amounts confer a health benefit. There are very limited studies on antidiabetic effect of lactic acid bacteria despite their potentiality in reducing the risk of diabetes onset. The present study explored the effect of administering live culture of   L. rhamnosus [MTCC No 8712] and L. casei [MTCC No 1408] in STZ induced diabetic albino male rats. Animals (approximately aged 6 months) were grouped into three in a randomized fashion that average weight of animals in each group would be 150-250 gms. Normal feed pellets and water ad libitum were provided to animals throughout the experiment. As  Group 1-Control Group, Group 2-Positive control Group were on only insulin treatment (1 unit /day) and the Group 3-Treated Group were on probiotics treatment.The treatment was continued for 30days. Body weight, blood glucose, lipid profile, antioxidative status was analyzed by standard protocol. After subculturing L. rhamnosus and L. casei and standardizing with Mac Ferlan’s device, the mixture culture was administered to the treatment group of animals by intraperitoneal injection ( 73×10⁸ cfu/g). With respect to body weight of the three groups after the treatment period, the treated group was found to have a drastic reduction in the body weight. While before treatment there was no significant difference. Glucose levels after treatment in both the probiotic and insulin treated groups were similar and comparable. Administration of the selected LAB Species L. rhamnosus and L. casei ameliorated glycemic status of the treated animals. Only Glutathione levels was markedly reduced in positive control groups (p<0.001) (insulin treated) and there was only a substantial reduction in the GSH level of probiotic treated (p<0.05). There was a significant reduction in total cholesterol, TGL, VLDL (P<0.001) in the probiotic treated group after treatment whereas there was no impact on LDL, HDL concentration. Hence, LAB strains of Lactobacillus rhamnosus and Lactobacillus casei may represent potential therapeutic age in reducing glycemic level, body weight and lipid profile but not much on antioxidative status except preventing considerable depletion of GSH.