IN-SILICO HOMOLOGY MODELING OF CRUSTIN PROTEIN IN PORTUNUS TRITUBER-CULATUS CRAB SPECIESAbstract
The aim of the present In-silico studies is to find out the potential protein target and find out the 3D structure of crustin antimicrobial protein in Portunus trituberculatus. Crustin are small cationic proteins assumed to be antimicrobial effectors against mainly Gram-positive bacteria. The swimming crab Portunus trituber-culatus is a commercially important crab species in East Asia countries. Gonadal development is a physiological process of great significance to reproduction and commercial seed production for P. trituberculatus. The nucleotide and protein sequence of crustins protein from P. trituberculatus is retrieved from the NCBI database in Fasta format. Further, the primary and functional analysis of crustin protein was done through protparam and helix turn helix tools. The retrieved protein sequence was applied to an advanced protein modeling server Cph to deliver the 3D structure. After modeling, the protein’s three Dimensional structure was viewed with Discovery studio software’s help. Our method enables the production of new antimicrobial peptides as potential next-generation antibiotics.
S. Moganapriya and K. Shoba *
Department of Biochemistry and Biotechnology, D. K. M. College for Women (Autonomous), Vellore, Tamil Nadu, India.
25 February 2022
15 June 2022
20 June 2022
01 October 2022