IN VITRO ANTHELMINTHIC AND ANTIOXIDANT ACTIVITY OF ISOLATED COMPOUNDS FROM ANNONA SQUAMOSA BARK

Objective: The present investigation of chemical constituents of Annona squamosa was undertaken as part of a wider study to find out the pharmacological active constituents present in this plant. So in this study, we investigate the pharmacological evaluation of previously isolated compounds (AS1-AS5) from the bark of Annona squamosa. Methods: The anthelminthic and antioxidant potential of the isolated compounds (AS-1 to AS-5) from Annona squamosa were evaluated for in-vitro anthelmintic activity using Indian earthworm Pheretima posthuma and DPPH model respectively. Results: The in-vitro anthelmintic activity results indicated that isolated compounds (AS-4 and AS-5) have good paralysis time comparable with albendazole standard. The antioxidant activity is evaluated for in-vitro antioxidant activity using DPPH radical scavenging activity, AS-5 & AS-4 having IC₅₀ 65.77 & 92.97 µg/ml revealed potent antioxidant activity comparable to standard ascorbic acid (IC₅₀ 43.42 µg/ml). Conclusion: This study suggests that bark of Annona squamosa have bioactive compounds for a new anthelmintic and antioxidant drug development. Further research on this plants core is needed for the discovery of a potent anthelmintic and antioxidant agent.